HPLC and LC-MS Overview

These flashcards cover the key concepts and terminology related to HPLC and LC-MS based on the lecture notes.

The key factors in HPLC are the mobile phase, the stationary phase and the __.

detector

In normal phase HPLC, the stationary phase is __ than the mobile phase.

more polar

In reverse phase HPLC, the stationary phase is __ than the mobile phase.

less polar

Mobile phases in normal phase HPLC include solvents like and .

dichloromethane, hexane

Hydrophobic analytes will be retained more strongly by the __ phase.

stationary

For analytes below 3,000 molecular weight, use a pore size of __ Å.

≤ 100

Higher surface area in HPLC leads to __ resolution.

more

The two ways of carrying out an HPLC experiment are isocratic elution and __ elution.

gradient

In HPLC-MS, the most common ionisation technique used is __.

electrospray ionisation (ESI)

The mass analyser is the region of the instrument where ions are separated according to their __ ratio.

m/z

Mass accuracy is how close the measured value is to the __ value.

true

In HPLC, the peak area is proportional to the amount of __ injected onto the column.

analyte

Quantitation can be performed using a __ curve constructed from known standards.

calibration

The two types of UV detectors are dispersive and __.

diode array

Electrospray ionisation generates __ charged ions from high molecular weight materials.

multiple