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Heat & UV
Vibrations disrupt hydrogen bonds & hydrophobic interactions
Detergents
Disrupt hydrogen bonds, hydrophobic interactions & salt bridges
PH change
Break hydrogen binds & disrupt ionic bonds and salt bridges
Organic compounds
Disrupt side-chain intra-molecular hydrogen bonding
Heavy metals
Denature proteins by forming bonds with ionic AA residues or reacting with disulfide -s-s- bonds
Agitation
Stretching of polypeptide chains until stabilizing interactions are disrupted