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In a Micropipet how much is 1uL in ml
1000ul
Explain how you dilute 1 mg/ml Methylene blue solution by a 1:20 dilution in a 10 ml final volume. Also, what is the final concentration of Methylene blue after the dilution?
provide the stock, dilvent, final concentration
stoc= fina v/ dilution (0.5)
dilvent: final v - stock (9.5)
final conce: starting conc/: (0.05)
what size can they go up to?
P20:
P200:
P1000:
0.5-20ul
20-200ul
200-1000ul

fill the following
1.5, 20, 200
15, 200, 1000
why do we need too read the absorbance immediately?
prevent change in concentration
why do we use a new pippet?
prevent contamination and prevent change in concentration