Final exam lab microbio

this selective plate has no differential substrate

phenylethyl alcohol agar

what carbohydrate is tested for fermentation in MacConkey Agar

lactose

The selective plate Columbia CNA contains what 2 antibiotics?

colistin & Nalidixic Acid

What is alpha hemolysis?

olive green characteristics due to partial breakdown of the hemoglobin

The selective ingredient in Mannitol Salt Agar, & P-ID of this test (yellow colonies)

staphylococcus aureus

What is the end product of interest for the coagulase test?

fibrin clot

The Bacitracin susceptibility test is useful in differentiating between members of this genus.

streptococcus

This is the P-ID of an organism for a Novobiocin susceptibility test with a zone of inhibition smaller than 16 mm (resistant).

staphylococcus saprophyticus

The VP test (part of MRVP) can identify this fermentation pathway if positive

2, 3-butanediol fermentation

What is the end product of interest (EPI) for the catalase test?

oxygen gas = bubbles!

What enzyme in the ETC, protons return through and produces ATP?

ATP synthase

what important product was discovered from penicillium notatum species?

penicillin

[2 part] The Plasmodium spp. parasite causes this disease. Also, these are the vectors of this parasite.

malaria, the female mosquito (anopheles), and humans

What form and compound is the substrate for the oxidase test, and what form and compound is the EPI?

• substrate: reduced form of TMPD

• EPI: oxidized form of TMPD

What type of antimicrobial inhibits growth of bacteria, allowing immune system to respond? Typical of protein synthesis inhibitors.

bacteriostatic

What variables are kept constant during the kriby-bauer antibiotic tests?

• pH

• agar depth

• incubation paramenters

• bacteria density (turbidity)

• inoculation method (lawn)

What term is used for an antibiotic that targets a wide range of bacteria?

broad-spectrum antibiotic

The β-lactam penicillin has this general mechanism of action.

cell wall synthesis inhibitor

What MOA’s do antibiotics Nalidixic acid and Ciprofloxacin have?

Inhibition of DNA gyrase, necessary for relaxation of DNA double-helix