3. Osmolality, Concentration, Duration of fixation

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Last updated 2:25 PM on 5/1/26
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11 Terms

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Best results are obtained using

slightly hypertonic solutions (400-450 mOsm)

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Hypertonic solutions =

Shrinkage

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Isotonic (340 mOsm)/Hypotonic solutions

swelling and poor fixation

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Added to Osmium tetroxide fixatives for EM

Sucrose

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Concentration of Formaldehyd

10%

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Glutaraldehyde

3%

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Glutaraldehyde for if you Immunoelectron microscopy

0.25%

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Duration of fixation for most formalin fixatives

24 hours (washed out)

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Duration of fixation for Buffered Formalin:

2-6 hours up to one week

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Duration of fixation for Electron Microscopy

3 hours

New books: 0 to 4 hours

Average: 2 hours

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Prolonged fixation may cause

Shrinkage and hardening of tissue