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Robert Hooke
Published Micrographia, coined the term cell
Antonie van Leeuwenhoek
"Discoverer of the microbial world", Described hundreds of moving particles he called animalcules and first description of bacteria
Aristotle
Created the term spontaneous generation
Louis Pasteur
Refuted spontaneous generation using "swan neck flask" experiment , developed pasteurization and proposed germ theory
Joseph Lister
Created sterile surgeries and developed the practice of antisepsis
Robert Koch
Formalizes standards to identify germs with infectious disease and developed a set of four postulates for isolating the causative agent of disease
Edward Jenner
"Father of Vaccination" and developed vaccination in inoculating individuals with the safer cowpox virus
Paul Ehrlich
Developed 606 "magic bullet" to cure syphilis
Alexander Fleming
Created penicillin through mold
Emerging diseases
Disease that are new to the population such as AIDS, SARS & MERS
Reemerging Diseases
Diseases that were under control in the past but are showing a resurgence in the present
Bioterrorism
Intentional or threatened use of biological agents to cause fear or inflict death/disease. Can be spread widely through the air and water to affect large populations.
Thermophilic
40-90C Needs a high temp to grow
Psychrophilic
0-20C Needs a very cold temp to grow
Halophilic
Need a high salt concentration to grow
Acidophilic
Needs an acidic temperature to grow
Anaerobic
Can live without O2
Aerobic
Can live with O2
Facultative
Can live with or without O2
Obligate intracellular parasites
Needs to be inside of a cell to grow (ex. viruses)
Quorum sensing
Cells can communicate with each other biochemically
Resolution
Most important part of a microscope; Seeing two distinct objects
Magnification
The process of enlarging an object in appearance
Lag phase
Cells metabolically active, no division yet.
Log phase (exponential phase)
Rapid cell division; most growth occurs here; antibiotics work the the best here
Stationary phase
Nutrients limited; growth = death. Stays the same
Death phase
Cells die off
Simple Stain
Staining with a basic dyes that are positive
Negative Stain
Staining with acidic dye which is negative and stain the background
Gram Stain
Most important stain in micro; Crystal violet (basic dye) then iodine (mordant) then 95% ethyl alcohol then Safranin (acidic dye)