6. organisms respond to changes in their internal and external environments

give the stages of urine formation:

1. formation of glomerular filtrate

2. reabsorption of glucose and water by proximal convoluted tubule

3. maintainance of a Na⁺ gradient in the medulla by the loop of Henle

4. reabsorption of water by distal convoluted tubule and collecting ducts

antidiuretic hormone (ADH) binds to V receptors found in cell-surface membranes in 2 parts of a nephron - name the 2 parts of a nephron where V receptors are found (1)

distal convoluted tubule and collecting duct

a decrease in blood pressure stimulates the release of ADH - give the location of the receptors that detect a decrease in blood pressure and explain how the release of ADH will affect blood pressure (3)

can you label this diagram of a nephron?

PCT = proximal convoluted tubule - closest to Bowman’s capsule

DCT = distal convoluted tubule - further from Bowman’s capsule

can you label this diagram of the kidney?

what does it mean if the loop of Henle is longer/medulla is deeper?

• lower ψ in medulla so Na⁺ gradient maintained for longer

• more water reabsorbed from collecting duct/end of DCT by osmosis

describe the formation of glomerular filtrate:

• blood enters the glomerulus through the afferent arteriole

• blood leaves the glomerulus through the efferent arteriole, which is smaller so maintains a higher hydrostatic pressure

• this high pressure forces smaller molecules e.g. water, glucose, urea out through gaps (fenestrations) in the capillary endothelium - this is ultrafiltration

• the molecules move through the basement membrane, which acts as a selective filter - proteins too large to pass through into the Bowman’s capsule

• smaller molecules move through the Bowman’s capsule epithelium through podocytes

• filtered fluid collects in the Bowman’s capsule

describe and explain how 3 features of the cells in the proximal convoluted tubule allow the rapid reabsorption of glucose into the blood (3)

give 4 adaptations of the distal convoluted tubule:

• many microvilli so SA increases

• many mitochondria so higher R rate

• ADH receptors

• selective permeability

what is the purpose of the distal convoluted tubule?

• makes final adjustments to pH balance and water content in the blood

• adjusting water reabsorption determined by ADH

describe the reabsorption of glucose and water in the proximal convoluted tubule:

• Na-K pump actively transports 3Na⁺ out of the epithelial cell into the capillary - this creates a concentration gradient of Na⁺ into the epithelial cell as the Na⁺ conc in the cells lining the PCT decreases

• glucose and Na⁺ enter the epithelial cell by facilitated diffusion - this is cotransport

• glucose diffuses out of the epithelial cell into the capillary by facilitated diffusion and is transported in the blood

what happens to urine if the ψ of the blood decreases?

• change detected by osmoreceptors in hypothalamus which shrink

• posterior pituitary gland secretes more ADH

• collecting duct and DCT membrane water permeability increases due to addition of aquaporins into plasma membranes

• smaller vol of less dilute urine as more water reabsorbed by osmosis from DCT/collecting duct

what happens to urine if the ψ of the blood increases?

• change detected by osmoreceptors in hypothalamus which expand

• posterior pituitary gland secretes less ADH

• so water permeability of collecting duct and DCT membrane increases as fewer aquaporins inserted in plasma membranes

• larger vol of more dilute urine as more water reabsorbed by osmosis from collecting duct/DCT

describe how the loop of Henle maintains a Na⁺ gradient:

• Na⁺ ions enter and water leaves descending limb by osmosis into interstitial fluid as descending limb permeable to water

• lowest ψ at tip of medulla - water reabsorbed by surrounding capillaries by osmosis

• at the bottom of the ascending limb, which is impermeable to water, Na⁺ and Cl^- diffuse out due to low concentration of filtrate - this increases the concentration of ions in interstitial space so ψ very low

• at top of ascending limb, Na⁺ and Cl^- leave by active transport and ion concentration in filtrate decreases as it ascends

• water remains in ascending limb because its walls are impermeable to water

where is the loop of Henle? describe its function:

• found in medulla - maintains Na⁺ grad

• ensures that urine produced is more concentrated than blood

• functions as a countercurrent multiplier - creates conc grad in surrounding medulla

describe the reabsorption of water by the distal convoluted tubule and collecting ducts:

• hypothalamus detects low water potential in blood and produces ADH which is secreted into the blood by the posterior pituitary gland

• ADH binds to receptors on cells lining the collecting duct on lumen

• causes vesicles containing aquaporins to be inserted into cell membrane

• water enters cell through aquaporins by osmosis down ψ grad, then moves by osmosis from cell to capillary via interstitial fluid

some people who have diabetes do not secrete insulin. explain how a lack of insulin affects reabsorption of glucose into the kidneys of a person who does not secrete insulin (4)

any 4 from:

• high concentration of glucose in the blood

• high concentration of glucose in the filtrate

• reabsorbed by co-transport (MS: facilitated diffusion/active transport)

• requires proteins/carriers

• these are working at maximum rate/saturated

• not all glucose is reabsorbed/some is lost in urine

some desert mammals have long loops of Henle and secrete large amounts of antidiuretic hormone (ADH). explain how these 2 features are adaptations to living in desert conditions (6)

a diabetic person and a non-diabetic person each ate the same amount of glucose. one hour later, the glucose concentration in the blood of the diabetic person was higher than that of the non-diabetic person - explain why (3)

in the diabetic person:

• lack of insulin/reduced sensitivity of cells to insulin

• reduced uptake of glucose by cells/liver/muscles

• reduced conversion of glucose to glycogen

the urine of a non-diabetic person does not contain glucose - explain why (2)

• leaves blood at kidney

• taken back into blood/reabsorbed (at kidney tubule)

• (reabsorbed) in proximal (MS: 1st) convoluted tubule

a high blood glucose concentration could cause glucose to be present in the urine of a diabetic person - suggest how (2)

• large amount/high concentration of glucose in filtrate

• cannot all be reabsorbed/proximal (MS: 1st) convoluted tubule too short to reabsorb all glucose/saturation of carriers

a test for glucose in urine uses immobilised enzymes on a plastic test strip. one of these enzymes ig glucose oxidase. explain why the test strip detects glucose and no other substance (2)

• enzyme has specific shape to active site/active site has specific 3^o structure

• only glucose fits/has complementary structure/can form E-S complex

if the glomerular filtrate of a diabetic person contains a high concentration of glucose, he produces a larger volume of urine - explain why (3)

• glucose in filtrate lowers water potential

• lower water potential/less difference in water potential filtrate/plasma

• less water reabsorbed by osmosis

can you label this diagram?

yes :)

why does the heart need to contract from the base upwards?

• contraction starts from the apex of the heart to move blood upwards to arteries, out of the ventricles

• empties as much blood as possible from the ventricles

what does it mean for the heart’s contraction to be myogenic?

the heart beats at a baseline rate w/o any input from the nervous system

what is the function of the sinoatrial node (SAN)? where is it located?

• sends a wave of electrical activity across the atria, depolarising it, causing atrial contraction (i.e. acts a pacemaker)

• located in the wall of the right atrium

what is the function of non-conducting collagen tissue?

prevents ventricles contracting at the same time as the atria

what is the function of the atrioventricular node (AVN)?

• delays electrical activity, allowing atria to fully empty

• sends a wave of electrical activity down the bundle of His and up the Purkyne fibres, depolarising them and causing the ventricles to contract from the apex upwards

explain why there is a short delay between the impulses generated by the SAN and those passing through the AVN (2)

• allows atria to contract and empty blood

• before ventricles contract

what is the autonomic nervous system?

• part of the nervous system which controls involuntary activities

• e.g. heart rate, blood pressure, digestion

• / ed into sympathetic and parasympathetic nervous system

name and explain the divisions of the autonomic nervous system:

• sympathetic:

  • stimulates effectors (i.e. increases heart rate)

  • speeds up activity

  • (aka. ‘fight/flight’)

• parasympathetic:

  • inhibits effectors (i.e. decreases heart rate)

  • slows down activity

  • (aka. ‘rest/digest’)

• sympathetic and parasympathetic nervous system are antagonistic - this means they have opposite effects at target tissues

which part of the brain controls changes to the heart rate?

medulla oblongata

what are baroreceptors? where are they located?

blood pressure receptors - located in walls of aortic and carotid arteries

what are chemoreceptors? where are they located?

chemical receptors - located in walls of aortic and carotid arteries

what happens when blood pressure increases?

• baroreceptors in aortic and carotid body are stretched

• baroreceptors send increased freq of nerve impulses to medulla oblongata

• increased freq of impulses and so more depolarisation across sympathetic pathway and stimulation of the SAN by acetylcholine

• decreased freq of waves of electrical activity spread across atria and ventricles to decrease heart rate

what happens after increased muscular/metabolic activity?

• increased muscular/metabolic activity → increased rate of resp

• CO₂ conc increases (O₂ conc decreases), causing pH of blood to decrease and H⁺ conc to increase

• chemoreceptors in the walls of aortic and carotid arteries detect the decrease in pH

• increase in freq of impulses and so more depolarisation to the medulla oblongata to increase heart rate

• increased freq of impulses along the sympathetic pathway to the SAN and noradrenaline is secreted

• an increased freq of waves of electrical activity spread across the atria and ventricles to increase heart rate

describe the myogenic stimulation of the heart and how the regular contraction of the atria and ventricles is coordinated - do not include the autonomic nervous system in your answer (5)

1. SAN releases (wave of) electrical activity

2. (so) atria contract (at the same time)

3. AVN relays/passes electrical activity after a (short) delay

4. (via) Purkyne tissues in/and bundle of His

5. (so) ventricles contract (at the same time from bottom upwards)

name the cells in the pancreas and give their function:

islets of Langerhans = clusters of specialised cells:

• alpha cells - secrete glucagon

• beta cells - secrete insulin

why is it important to regulate blood glucose concentration in the bloodstream?

extreme blood glucose levels cause changes in water potential, potentially causing cell lysis

what is glycogenesis? when does it occur?

• literally: glycogen synthesis

• conversion of glucose → glycogen in condensation reactions

• occurs when blood glucose conc is higher than normal

describe what happens when blood glucose conc is too high:

• beta cells in the pancreas detect high blood glucose levels, secreting insulin into the bloodstream

• insulin binds to receptors on target cells

• there are more glucose channel proteins in the target cell membrane, increasing permeability, as vesicles containing these proteins fuse w/ the membrane so more glucose diffuses into the target cells

• insulin also activates enzymes that convert glucose → glycogen via condensation reactions in glycogenesis

• the glucose concentration in cells decreases, creating a diffusion gradient for more glucose to diffuse in, decreasing the glucose concentration in the blood

what is glycogenolysis?

• literally: glycogen hydrolysis

• hydrolysis of glycogen → glucose

• occurs when blood glucose conc is too low

what is gluconeogenesis?

• literally: glucose new synthesis

• conversion of AAs and lipids → glucose

• occurs when blood glucose conc is too low

describe what happens when blood glucose levels are too low:

• alpha cells in pancreas detect low blood glucose conc and secrete glucagon

• glucagon binds to specific protein receptors on the surface membranes of liver cells

• activates enzymes that hydrolyse glycogen → glucose in glycogenolysis

• this activates enzymes that convert AAs and lipids → glucose in gluconeogenesis

• glucose then leavers the liver cells by FD, increasing glucose conc in blood

describe how adrenaline acts via the second messenger model:

• secreted by adrenal glands to increase blood glucose conc during times of excitement/stress

• adrenaline binds to a complementary receptor on the cell surface membrane of a liver cell

• the binding of adrenaline causes the receptor to change shape, activating a G protein

• this activates the enzyme adenylyl cyclase

• the activated adenylyl cyclase converts ATP → cAMP

• cAMP acts as a 2nd messenger, binding to and activating many protein kinases via phosphorylation, amplifying the signal from adrenaline

• protein kinases activate enzymes that catalyse the breakdown of glycogen into glucose in glycogenolysis

• glucose moves out of liver cells by FD and into the blood through channel proteins

• this increases blood glucose conc so more glucose can be delivered to body cells for R

in the second messenger model, what are the first and second messengers? how do they affect each other and what is the rseult?

• 1st messenger = hormone e.g. adrenaline triggers the formation of the 2nd messenger

• 2nd messenger = cAMP activates enzymes to carry out extracellular signalling

what is an action potential?

rapid impulse that travels along a neurone, causing changes in membrane potential

what are the 3 main stages of generating an action potential?

1. depolarisation

2. repolarisation

3. hyperpolarisation

describe and explain how an action potential is generated:

1. resting neurone at resting potential - all Na⁺ channels are closed

2. stimulus arrives at neurone, causing voltage-gated Na⁺ channels to open ∴ Na⁺ diffuses into the axons down an electrochemical gradient, making it less -ve

3. if the membrane potential reaches the threshold potential of -55 mV, more voltage-gated Na⁺ channels open - this influx of Na⁺ causes depolarisation

4. when enough Na⁺ enters the axon, membrane potential reaches +40 mV - this is action potential

5. when action potential has been reached, all voltage-gated Na⁺ channels close and voltage gated K⁺ channels open - this means that K⁺ diffuse down the electrochemical gradient out of the axon

6. the diffusion of K⁺ out causes a temporary overshoot of the resting potential - hyperpolarisation - as part of the refractory period

7. to restore resting potential, voltage-gated K⁺ channels close and Na-K pump actively transports 3Na⁺ out and 2K⁺ in

what is depolarisation?

a reversal in membrane potential

what voltage is action potential?

+40 mV

what voltage is the threshold potential?

-55 mV

how does the action potential move along the neurone?

as a wave of depolarisation

how does the speed of action potential transmission change with axon diameter?

• larger axon diameter means there is less resistance to ion flow

• ∴ wave of depolarisation travels faster

how does the speed of action potential change with temperature?

• higher temp → faster diffusion of ions

• ∴ faster action potential transmissions

• over 40^oC - proteins denature → slower action potential transmission due to membrane damage

explain the importance of the refractory period:

• ensures action potentials are discrete (i.e. don’t overlap)

• limits the freq of impulses by setting a minimum time period between action potentials

• ensures impulse travels in 1 direction

describe and explain the all-or-nothing principle:

• once the threshold is reached, an action potential will always fire w/ the same change in voltage, no matter how big the stimulus is

• if the threshold isn’t reached, an action potential won’t fire

• a bigger stimulus won’t cause a bigger action potential, but it will cause them to fire more frequently

complete this table:

A = closed

B = open

C = closed

D = some are open

E = closed

F = open

describe how resting potential is maintained:

• Na⁺ channels are voltage gated and closed to prevent Na⁺ diffusing into the neurone

• membrane more permeable to K⁺ ions and less permeable to Na⁺ ions

• K⁺ leak channels allow FD of K⁺ out of the neurone

• Na-K pump actively transports 3Na⁺ out and 2K⁺ into the neurone

• this forms an electrochemical gradient as +ve ions accumulate in the extracellular space

• this makes the axon cytoplasm -vely charged so the membrane is polarised

what is the function of the Na-K pump?

• actively transports 3Na⁺ out and 2K⁺ into the neurone

• restores resting potential after action potential

what is the resting potential?

diff in electrical charge across cell surface membrane when a neurone is not transmitting an impulse - 70 mV (the inside of an axon has a charge that is 70 mV more -ve than the outside)

give and explain the features of this neurone:

• cell body - contains nucleus and other organelles e.g. mitochondria and ER

• dendrons - short branches extended from the cell body which further / into dendrites

• axon - single nerve fibre which carries impulse away from cell body to other neurones/effectors

give the order of travel for a reflex arc:

sensory neurone → relay neurone (in CNS)→ motor neurone → effector

what are the functions of Schwann cells?

• membranes form myelin sheath

• remove debris via phagocytosis

• aid regeneration

suggest 2 advantages of simple reflexes:

any 2 from:

• rapid

• protect against damage to body tissues

• do not have to be learnt

• help escape from predators

• enable homeostatic control

in this investigation, it is not possible for a student to react in less than 45 ms - suggest one explanation for the value recorded in Trial 3 in Table 1 (1)

in response to touch, nerve impulses can be transmitted at speeds of 76.2 m s^-1 - suggest 3 reasons why in this investigation, the estimated speed of student A’s impulse transmission was less than 76.2 m s^-1 (3)

a scientist investigated the effect of inhibitors on neurones. she added a respiratory inhibitor to a neurone and the resting potential of the neurone changed from -70 mV to 0 mv - explain why (3)

explain why different proteins are required for the diffusion of different ions through the membrane (2)

• each protein has a specific 3^o structure

• diff ions have diff structures/shapes

describe how the change shown in the diagram occurs when an action potential is produced (2)

• Na⁺ channels open

• Na⁺ ions enter the axon

explain what causes the conduction of impulses along a non myelinated axon to be slower than along a myelinated axon (3)

• myelinated - ion movement only at nodes of Ranvier

  • impulse jumps from node to node via saltatory conduction

• non myelinated - more depolarisation over whole length of neurone and no saltatory conduction

what is a synapse?

junction between 2 neurones

can you label the parts of the synapse?

describe the process of synaptic transmission:

1. at a cholinergic synapse, an action potential arrives at the presynaptic membrane, causing it to depolarise

2. this triggers the opening of Ca²⁺ ion channels, allows Ca²⁺ ions to enter the pre-synaptic knob by FD

3. the influx of Ca²⁺ ions causes synaptic vesicles containing the neurotransmitter acetylcholine to move towards and fuse w/ the presynaptic membrane

4. the neurotransmitter is then released into the synapse/synaptic cleft - it diffuses along the gap and binds to specific receptors on the post-synaptic membrane

5. this binding causes Na⁺ ion channels to open, allowing Na⁺ ions to diffuse into the post synaptic neurone

6. if enough Na⁺ ions enter, the membrane will reach the threshold, causing depolarisation and generating a new action potential

what is acetylcholinesterase?

• enzyme which catalyses the hydrolysis of acetylcholine into acetate and choline

  • these products are reabsorbed (endocytosis) into the presynaptic neurone where acetylcholine is regenerated using E from ATP

• if neurotransmitter not removed, keeps binding to receptors and keeps producing action potentials

is the cholinergic synapse stimulatory or inhibitory?

stimulatory

give an example of an inhibitory neurotransmitter and describe what happens at an inhibitory synapse:

e.g. GABA:

• inhibitory neurotransmitters released into the synaptic cleft and bind to Cl^- channels on the postsynaptic membrane

• Cl^- channels open, allowing an influx of Cl^- into the postsynaptic neurone by FD

• (K⁺ channels open, allowing K⁺ ions to leave the postsynaptic neurone)

• → the postsynaptic membrane is hyperpolarised, so action potential not produced and depolarisation does not occur

• ∴ more sodium ions required to reach threshold for depolarisation/action potential

can you fill in this table?

what is summation?

the process in which the effects of multiple neurotransmitters are combined to produce a response

describe and explain temporal summation:

repeated impulses in short succession from the same presynaptic neurone provide enough acetylcholine for the threshold to be reached

in temporal summation, why must the repeated firing occur in short succession?

if the second firing does not occur until a while after the first firing, the action potential from the first firing gets broken down (as it does not reach the threshold)

describe and explain spatial summation:

• multiple presynaptic neurones converge on 1 postsynaptic neurone

• individually, the neurones do not release enough neurotransmitter to reach the threshold

• but the combined effect of all neurotransmitters is enough for the postsynaptic neurone to reach the threshold and trigger an action potential

why may summation occur when an inhibitory synapse is present?

• if the threshold is reached, an action potential will fire

• if multiple presynaptic neurones converge on 1 postsynaptic neurone and some are excitatory and others are inhibitory:

  • for an action potential to occur, the excitatory neurones must summate

  • to overcome the hyperpolarisation caused by the inhibitory neurones

S is a similar shape to acetylcholine - suggest how anaesthetic S stops the transmission across the synapse (3)

• complementary to receptor for acetylcholine

• binds to receptor

• on postsynaptic membrane

• prevents acetylcholine from binding

• ∴ no action potential in postsynaptic neurone as neuronal activity is inhibited

give 2 key features of receptors:

• receptors respond only to specific stimuli

• stimulation of a receptor leads to the establishment of a generator potential

what is a generator potential?

the initial nervous impulse that is generated

can you label this Pacinian corpuscle?

• (sensory neurone) axon

• (sensory) neurone ending

give an example of a receptor - what does it respond to and what does it consist of?

• mechanoreceptor found in the skin

• responds to pressure/vibrations

• consists of the end of a sensory neurone wrapped in layers of connective tissue

what are stretch-mediated ion channels? what do they respond to?

• ion channels present in Pacininian corpuscles - Na⁺

• respond to mechanical forces along the plane of the cell membrane (membrane tension) but not to hydrostatic pressure perpendicular to it

describe what happens when the Pacinian corpuscle is stimulated:

• pressure causes the lamellae to become deformed

• increase in pressure deforms the stretch mediated Na⁺ ion channels in the sensory neurone’s plasma membrane

• Na⁺ ion channels in membrane open

• Na⁺ ions diffuse in, depolarising the nerve ending

• this leads to a generator potential, which if the threshold is met, generates an action potential

• the increase in pressure causes more Na⁺ channels to open so more Na⁺ ions can enter

give the order of connections between photoreceptors and the CNS:

rods and cones (synapses) bipolar neurones → ganglion cells → optic nerve → CNS

what are the 3 types of cone cells?

• red sensitive cone cells

• green sensitive cone cells

• blue sensitive cone cells

where are the photoreceptors found on the eye? describe and explain their distribution:

in the retina:

• rod cells - across entire retina except fovea

• cone cells - on fovea

  • as cone cells only respond to high light intensities and the fovea receives the highest intensity of light as this is where the lens focuses light

  • this means that rod cells can be located further from the fovea as they can respond at lower light intensities

compare and contrast rod and cone cells:

• rod cells found across entire retina except fovea, whereas cone cells only found on fovea

• rod cells highly sensitive to light, whereas cone cells are less sensitive to light

• rod cells can only generate B&W images, whereas cone cells can generate images in colour

• rod cells provide low visual acuity, whereas cone cells provide a higher visual acuity

• the optical pigment in rod cells is rhodopsin, whereas the optical pigment in cone cells is iodopsin

how do rods and cones detect light?

• light causes the chemical breakdown of optical pigment inside rods and cones

• change in membrane potential causes Na⁺ to diffuse in, establishing a generator potential

• if the generator potential reaches the threshold then an action potential is sent along a bipolar neurone to the optic nerve

can rod cells detect light at very low intensities? why?

yes! retinal convergence/high sensitivity - many rod cells connect to 1 neurone so the threshold is more likely to be reached as spatial summation occurs so enough neurotransmitter

do rod cells have a high or low visual acuity?

low: many rod cells connect to 1 bipolar neurone - the brain is unable to distinguish between separate light sources as multiple signals are sent to the brain

do cone cells have high or low visual acuity? why?

high:

• 1 cone cell connects to 1 neurone, sending separate impulses to the brain

• this allows points close together to be distinguished

can cone cells detect light at very low intensities? why?

no:

• cone cells connected to 1 bipolar neurone

• threshold unlikely to be reached to produce an action potential