micro lab FINAL TEST NOW pt.1
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78 Terms
what is this?
MacConkey Agar
what is the ph indicator?
neutral red
what is it used for?
clinical samples
what type of agar is it?
selective and differential agar
what is the selective agents?
bile salts and crystal violet
what does it inhibits?
Gram positive bacteria
what is the differential agent?- MAC
lactose
why is it red? did it ferment lactose yes or no
it ferments lactose
why is it white?
does not ferment lactose
if its red is it + or -?
positive
name a positive bacteria that is red?
e.coli
name a bacteria that turn white?
Salmonella
if theres no growth its? + or -
Gram positive
what is this?
eosin methylene blue agar
does it have a ph indicator?
No
what is it used for?-emb
water samples
what type of agar?
selective and differential agar
what are the selective agents?
eosin, methylene blue, and crystal violet
who does it inhibit?
Gram positive
who grows?
gram negative bacteria
differential agent?
lactose
if it’s green did it ferment lactose or not?
fermented lactose
a bacteria that is green and fermented lactose is?
E.coli
if it’s pink or red did it ferment lactose or no? emb
it fermented lactose
pink/ red bacteria? EMB
enterobacter
whats this?
eosin methylene blue agar
what does it mean if it’s colorless but it still grows?
did it or did it not ferment lactose?
it does not ferment lactoses
what bacteria is colorless and doesn’t ferment lactose?EMB
salmonella
if there’s no growth its?
Gram positive bacteria
what’s this?
heckoin enteric agar
what’s the ph indicator?
bromothymol blue
what’s its used for?
for stool samples
what type of agar is this? HE
selective and differential agar
what are the selective agents?
bile salts
who does it inhibit?
Gram positive
who grows?
gram negative bacteria
whats the differential agent?…. and includes …., ….
Lactose and includes sucrose and salicin
what shows H2s production?
peptone iron
if it’s yellow orange did it ferment lactose or no ?
fermented lactose
what bacteriaq is it if it’s orange/ yellow?
e.coli
if it’s yellow’orange, black did it ferment lactose or no and did it produce h2s or no?
fermented lactose and produce h2s
what bacteria is it if it’s yellow/black?
citrobacter
if its the color of agar did it ferment or not lactose?
… …. …. … and bacteria name ….?
does not ferment lactose and alcaligenes
if it’s black its?… ….
h2s production
name a bacteria that has black production?
proteus
bottom arrow is what ? is there growth ?+ or -?
no growth, gram positive
what’s this?
oxidase test
what does it distuinguish?
non enteric gram positive bacteria and enteric gram negative bacteria
what does it test for? ….. ….. in …. …. ….. like ….
cytochrome oxidase in aerobic ETC pathway like mitochondria
reagent?
oxidase reagent
if its blue/ black color on swab its?
non enteric gram positive bacteria
if it’s not blue/black it’s?
enteric gram negative
if it’s blue/black what bacteria is it?
pseudomonas
if it’s not blue/black its what bacteria?
e.coli
what’s this?
decarboxylase test
what the top arrow pointing at?…. … it;s ….
mineral oil it’s anaerobic
what’s the ph indicator?
bromocresol purple
what are the substrate?
lysine, arginine, ornithine
what are the enzymes?
lysine decarboxylase, arginine decarboxylase, ornithine decarboxylase
product?
ammonia
what does the enzyme do? …. … … from … … making it ….
removes acid group from amino acid making it alkaline
if it’s purple with mineral oil its?
positive
if it’s yellow? + or - for…?
negative for decarboxylation
whos ORN decarboxylase positive?
enterobacter
who’s ARG decarboxylase positive?
salmonella
who’s LYS decarboxylase positive?
klebsiella
what’s this?
phenylalanine deaminase test
what’s the substrate?
phenylalanine
what’s the enzyme?
phenylalanine deaminase
what does the enzyme do?
removes amino group from phenylalanine into products
what are the products?
phenyl pyruvate and ammonia
what is the left side picture? color… with …. ….
green with ferric chloride
is the left picture + or - for ….. …..?
positive for phenyl pyruvate
left side bacteria GENUS name?
proteus
what are we testing for?
phenyl pyruvate
what’s right side picture?
yellow with ferric chloride
is it + or - for … …? right pic
negative for phenyl pyruvate
right side bacteria NAME?
e.coli