lab

Microscope

10X = 100X

30X = 300X

40X = 400X

100X = 1000X (oil immersion)

What is serum

Clear liquid that can be separated from clotted blood

Cytology

Study of cells, their origin, structure, function and pathology

What is plasma

Type of liquid separated from whole blood

Pathology

Study of the diseases of tissues and organs- the cause, how it develops, and how it affects the body

Disease that you should test before bringing a new cat into a household and why

Feline leukaemia FeLV/FIV

• Highly contagious

what body part is required for rabies testing

Brain

Common test to use with Diff-Quick

• ear cytology

• Blood film

Feathered edge

Area of slide where CBC is evaluated

Preferred test for fecal evaluation

Centrifugation (preferred for what test)

Blood cells present in the Buffy coat

WBC and thrombocytes

Thrombocytes

A type of platelet

Flocculent

cloudy fluid with chunks

Hematology

The study of blood and blood disease/disorders

Type of microscope used for feces and urine

Horizontal/swing arm

Buffy coat

thin white layer in hematocrit tube- Red blood cells and platelets

Where the PCV is determined

Amount of feces needed for fecal analysis

1-2 grams

Tapeworm proglottids

The parasite visible in gross examination of feces

Sample required for CBC

Unclotted whole blood (depending on test)

Most efficient method of fecal analysis for detection of parasites

centrifugation (most efficient method for)

NOT needed for fecal smear

Centrifuge (not needed for what)

Liquid NOT acceptable for fecal flotation

Distilled water

Culture test

Test that identifies organism that’s causing disease

Sensitivity test

Indicates which medicine would work

Bacillus

Oblong shaped bacteria (long)

Hemolysis

Rupture of red blood cells causing serum or plasma to be tinted red

Lipemia

Excess of lipids or fats in the blood

Lipids

Fat like substances

• triglycerides

• Cholesterol

• Phospholipids

• Fatty acids

• Lipoproteins

Triglycerides

The main blood fat measured in chemistry panels

Phospholipids

Part of cell membrane

Lipoproteins

HDL, LDL, VLDL

• transport fats in blood

Serum- what tube/additive

Red or SST

Plasma- how to prepare

Freeze then centrifuge

Whole blood for chemistry procedure- what tube/additive

Purple top EDTA

Microscope cleaning

Clean with lens paper and lens cleaner immediately after

Supernatant

liquid portion left over after centrifuging

Sediment/pellet

solid portion left over after centrifuging

Microhematocrit centrifuge/how and what used for

Used for PCV test

Uses the clay sealant

Refractometer- what can you measure in it

clean with?

Urine, serum, or plasma

Clean with distilled water

Routine blood chemistries

• Electrolytes

• Complete blood count (cbc)

• Samples of whole blood, serum or plasma

Serological test AKA

Also known as snap tests

Agglutination test

Type of snap test used to detect antibodies

Required adding antigen

preprandial

AC-Before eating

Postprandial

PC-After eating

Purple top tube

EDTA

anticoagulant

Green top

Lithium heparin

Anticoagulant

Tiger top

SST

clot activator

Gel in bottom

Red/white top

Empty tube

Blue top

Sodium citrate

Anticoagulant

Donut

Grey top

Sodium fluoride/potassium oxalate

Prevent glucose breakdown

Serum vs plasma

The coagulant/additive

TP (stands for)

total protein

What does aPTT stand for and what does it measure

Activated partial thromboplastin time

Measures how long it takes for blood to clot

CBC

Compete blood count

Evaluates RBC’s, WBC’s, and platelets

What is PCV

Packed cell volume

Also known as hematocrit

Capillary tubes

Separates RBC’s and plasma

Hematocrit- also known as

PCV

DTM

Dermatophyte test medium

Test performed on culture plate to evaluate fungus

Gram stain

Test to determine if bacteria species are present

Gram stain purple

Gram-positive

Gram stain pink

Gram-negative

Excisional sample

Small amount of tissue removed

Incisional sample

Entire area removed for testing

Gram stain bacteria

Rods or cocci

Cocci

Round shaped bacteria

Void sample

A sample that the animal excretes

• free catch

• Bladder expression

Necropsy

The examination of a dead animal