Laboratory

Miscibility

What does the solubility test detect?

Homogenous solution

All reagents prepared in solubility test are what kind of solution?

Ninhydrin test

detects amino acid and proteins

Oxidative deamination

In ninhydrin test what does amino acid undergo?

CO2, HN3, and an aldehyde with hydrindatin

Oxidative deamination results in the liberation of what?

Diketohydrin/ruhemann’s complex-blue

Ammonia react with another ninhydrin resulting in what?

Yellow

In ninhydrin test what is the complex’s positive color when proline is present?

Brown

Asparagine results in what color when ninhydrin test is used?

Amide group

Why is the result brown when asparagine is detected using ninhydrin test?

Deep blue or purple

Positive result of ninhydrin test

Xanthroproteic test

detects tyrosine and tryptophan

Dark yellow or orange

A positive xanthoproteic test is what color?

Sakaguchii test

Detects arginine

Red

A positive sakaguchii test is what color?

Acree-rosenheim test

Detects tryptophan

Purple ring

A positive acree-rosenheim test is what color?

Methionine

What does the mccarthy sullivan test detect?

Red

What color does a positive mccarthy sullivan test have?

Tyrosine

Million's test detects what?

Red or pink precipitate

A positive million’s test have what color?

Isotonic

A buffer is what in nature?

Electrophoresis

Process of separating nucleic acids and proteins based on their size and electric charge

Sodium dodecyl sulfate polyacrylamide gel electrophoresis

SDS-PAGE

SDS

Strong denaturing agent that attracts the negative proteins to the positive electrons

Polyacrylamide gel

Mesh-like matrix that separates proteins based on their size