IH LEC UNIT BLOOD COMPONENT

Hemotherapy

Use blood components to treat a disease in a patient

Component therapy

Transfusion of specific components rather than whole blood to treat a patient

Heavy spin

5000xg for 5 minutes

Heavy / Light Spin? (indication rcf)

Packed RBC

Heavy spin

5000xg for 5 minutes

Heavy / Light Spin? (indication rcf)

Platelet concentrate

Heavy spin

5000xg for 7 minutes

Heavy / Light Spin? (indication rcf)

Cryoprecipitate

Heavy spin

5000xg for 7 minutes

Heavy / Light Spin? (indication rcf)

Cell free plasma

Light spin

2000xg for 3 minutes

Heavy / Light Spin? (indication rcf)

Platelet-rich plasma

20-24 deg C

For the preparation of platelet concentrate, centrifugation is performed at what temperature?

1-6 deg C

For all other blood components, centrifugation is carried out between what temperature?

2-6 deg C

In sedimentation of packed RBC, stand overnight at what temperature?

20-40 um

Pore size of microaggregate filter

WBC

Donor plasma / WBC (can induce)?

Fever

WBC

Donor plasma / WBC (can induce)?

HLA antibody

WBC

Donor plasma / WBC (can induce)?

Virus

WBC

Donor plasma / WBC (can induce)?

Allergens

WBC

Donor plasma / WBC (can induce)?

IgA

WBC

Donor plasma / WBC (can induce)?

Anaphylactic reaction

Plasma proteins

Washing during blood component preparation removes?

Pre-chilled NSS

Washing uses what kind of NSS?

T

T/F?

Homemade NSS cannot be used in washing

T lymphocytes

Target of Irradiation

more than 20%

RBC is indicated if acute loss is _____________ blood volume

less than 6 g/dL

RBC is indicated if hemoglobin is ____________

less than 18%

RBC is indicated if hematocrit is ____________

Px hemoglobin is less than 8 g/dL and with acute coronary syndrome

RBC is indicated if there is acute loss of blood volume / less hgb / less hct UNLESS

1 to 1-1.5 g/dL

1 unit of WB or packed RBC can increase hemoglobin by?

3-5%

1 unit of WB or packed RBC can increase hematocrit by?

80%

In packed RBCs, how many percent of plasma is removed?

70-80%

Hematocrit of Packed RBCs

1 to 10 deg C

Transport temp Packed RBCs

48 hours

Leukocyte-reduced RBCs filtration is within _________ from the time of collection

Cesium 137 / Cobalt 60

Used gamma in Irradiated RBCs

25-35 Gy

Recommended minimum dose of gamma irradiation

Frozen RBCs are frozen in glycerol within ____________ of collection

High Glycerol

Slow freezing of RBC

Low Glycerol

Fast freezing of RBC

40% glycerol

Reagent used in slow freezing

20% glycerol

Reagent used in fast freezing

Glycerol, Glucose, Fructose, EDTA

Reagent used in Agglomeration of RBC

-80 deg C

Fast freezing frozen at what temp

-196 deg C

Slow freezing frozen at what temp

-80 deg C

Agglomeration of RBC frozen at what temp

Mechanical

Type of Freezer used in slow freezing

Mechanical

Type of Freezer used in agglomeration of RBC

Liquid Nitrogen

Type of Freezer used in fast freezing

-65 deg C

Slow freezing, RBCs stored at what temp

-120 deg C

Fast freezing (Low Glycerol) RBCs stored at what temp

-65 deg C

Agglomeratiom, RBCs stored at what temp

hypertonic solution

During deglycerolization, wash RBCs in what type of solution

isotonic solution

During deglycerolization, wash RBCs in a solution followed by what type of solution

12% NaCl --> 1.6% NaCl --> 0.9% NaCl + 0.2% dextrose

High glycerol steps

4.5% NaCl in 15% mannitol --> 0.9% NaCl

Low glycerol steps

50% glucose + 5% fructose --> 0.9% NaCl

Agglomeration

60-90 days

RBC life in Neocyte-enriched blood px

5,000 - 10,000 / uL

1 unit of random donor platelet should increase platelets by _________ in 75-kg recipient

20,000 - 6,000 / uL

1 unit of single donor platelet should increase platelets by _________ in 75-kg recipient

6-8 hours

Platelet concentrate or Random donor platelet requires centrifugation of Whole blood at room temp within _________ of collection

5 days

Shelf life of RDP and SDP

4 hours

Shelf life of platelet concentrate or random donor platelet after pooling

at least 5.5 x 10^10

1 unit of random donor platelet contains how much platelets?

50-70 mL

Volume of plasma in platelet concentrate

at least 6.2

pH platelet concentrate or random donor platelet

Apheresis

Single Donor Platelet (SDP) or Plateletpheresis Unit

Preparation

at least 3.0 x 10^11

1 unit of Single Donor Platelet (SDP) or Plateletpheresis Unit contains how many platelets?

300 mL

Volume of plasma in Single Donor Platelet (SDP) or Plateletpheresis Unit

at least 6.2

pH of Single Donor Platelet (SDP) or Plateletpheresis Unit

less than 8.3 x 10^5

Leukoreduced platelets must contain how much leukocytes?

<5 x 10^6 WBCs

Absolute WBC count in leukoreduced RBCs

4 hours

High glycerol freezing of RBCs must be placed in a freezer within how many hours of opening the system?

at least 4.0 x 10^9 /L

In Apheresis Granulocytes or Granulocyte Pheresis Unit, the donor must have an absolute granulocyte count of ___________

12-24 hours

In Apheresis Granulocytes or Granulocyte Pheresis Unit, administer corticosteroid to donor within _______ before donation?

Hydroxyethyl starch

Sedimenting agent used in Apheresis Granulocytes or Granulocyte Pheresis Unit

>1.0 x 10^11

typical therapeutic dose of granulocyte concentrate each day

5 days

typical therapeutic dose of granulocyte concentrate is

>1.0 x 10^10 each day for how many days

20-50 mL

Volume of RBCs in Apheresis Granulocytes or Granulocyte Pheresis Unit

21 days

Whole blood and RBC shelf life if with:

CPD

35 days

Whole blood and RBC shelf life if with:

CPDA-1

21 days

Whole blood and RBC shelf life if with:

CP2D

21 days

Whole blood and RBC shelf life if with:

ACD

42 days

RBC shelf life if with:

Adenine-saline

28 days

RBC irradiated shelf life

24 hours

RBC leukoreduced shelf life

Open system

24 hours

Washed RBCs shelf-life

10 years

Frozen RBCs shelf-life

24 hours

RBC deglycerolized shelf-life

5 days

Platelets RD, SD, Irradiated, leukoreduced shelf-life

4 hours

Platelets pooled shelf-life

1 year

FFP and PF24 shelf-life if -18 deg C

7 years

FFP and PF24 shelf-life if -65 deg C

1 yr

Cryoprecipitate shelf-life if frozen

6 hours

Cryoprecipitate shelf-life if thawed

4 hours

Cryoprecipitate shelf-life if pooled

24 hours

Granulocytes shelf life

2 years

RhIg shelf life

24 hours

Components stored at 1-6 deg C must be used within ____________ after the system opened

4 hours

Components stored at 20-24 deg C must be used within ____________ after the system opened

1-6 deg C

Storage temp of Whole Blood and RBCs (except Frozen)

20-24 deg C

Storage temp of random donor Platelets

-18 deg C

Storage temp of FFP and PF24 if stored for 1 yr

-65 deg C

Storage temp of FFP and PF24 if stored for 7yrs