MOLBIO LAB 6

What personal sources can contribute to contamination in the molecular laboratory

Hair, skin, saliva, and clothes

What is the most common source of contamination in a molecular diagnostics laboratory?

Aerosols

What type of contamination occurs when DNA from one specimen is accidentally transferred to another specimen?

Cross contamination

What type of contamination occurs when amplified PCR products contaminate other samples or surfaces?

Amplification product contamination

What laboratory design principle requires that the workflow moves only in a single direction to minimize contamination?

Unidirectional workflow

What are the three working areas in an ideal molecular diagnostics laboratory?

Reagent preparation area, Sample preparation area, Amplification detection area

What laboratory area is used for the storage of chemicals and preparation of PCR reagents or master mix?

Reagent preparation room (No template laboratory)

What type of air pressure is used in the reagent preparation room?

Positive air pressure

What laboratory area receives specimens and performs nucleic acid extraction?

Sample preparation area

What equipment is commonly used in the sample preparation area to prevent contamination while handling specimens?

Biosafety Cabinet Class II (BSC II)

What laboratory area is considered the "dirty room" where amplified DNA is handled?

Amplification detection area

What type of air pressure is used in the amplification detection room?

Negative air pressure

What instrument is commonly placed in the amplification detection room to perform PCR?

Thermocycler

What device provides a tightly controlled environment for reagent preparation?

Dead Air Box

What equipment is a larger working cabinet used for PCR reagent preparation?

PCR Cabinet

What small intermediate room separates laboratory areas and helps create differential air pressure?

Anteroom

What is the purpose of UV light in a dead air box?

Decontaminate surfaces

What disinfectant is commonly used to clean surfaces in molecular laboratories after UV treatment?

10% sodium hypochlorite

What solution is used after sodium hypochlorite to further clean surfaces?

75% ethanol

What laboratory area is also called the "clean room"?

Preamplification room

What laboratory area is also called the "dirty room"?

Postamplification room