energy, enzymes, mitochondria and respiration 4A, 4B, 4C

What is energy in biological systems?

Ability to do work in cells; required for all cellular processes

What are the forms of energy?

Kinetic (movement); thermal (heat); potential (stored); chemical (in bonds)

What is the first law of thermodynamics?

Energy cannot be created or destroyed, only converted between forms

What is the second law of thermodynamics?

Energy transfer increases entropy (disorder) of the universe

What is entropy?

Measure of disorder/randomness in a system

What is metabolism?

All chemical reactions in a cell or organism

What are catabolic reactions?

Break down molecules; release energy

What are anabolic reactions?

Build molecules; require energy

What is free energy (G)?

Energy available to do work in a system

What is ΔG?

Change in free energy during a reaction

What does ΔG = 0 mean?

System is at equilibrium

What does negative ΔG mean?

Exergonic; releases energy; spontaneous

What does positive ΔG mean?

Endergonic; requires energy input; non-spontaneous

What is an exergonic reaction?

Reaction that releases energy (ΔG < 0)

What is an endergonic reaction?

Reaction that requires energy (ΔG > 0)

What is an example of an exergonic reaction?

Cellular respiration (glucose breakdown)

What is an example of an endergonic reaction?

Photosynthesis

What is activation energy (EA)?

Energy required to start a reaction

Why do reactions need activation energy?

To reach transition state where bonds can break/form

What is ATP?

Adenosine triphosphate; main energy carrier in cells

What are the components of ATP?

Adenine; ribose; three phosphate groups

Where is energy stored in ATP?

Bonds between phosphate groups

How much energy does ATP release?

~30.5 kJ/mol per ATP

What happens during ATP hydrolysis?

ATP → ADP + Pi + energy

What is energy coupling?

Using energy from exergonic reactions to drive endergonic reactions

How does ATP drive endergonic reactions?

Transfers phosphate to substrate making it more reactive and increasing its energy so the reaction can proceed.

What is the ATP cycle?

ATP ↔ ADP + Pi (continuous regeneration)

What are enzymes?

Protein catalysts that speed up reactions without being used up

What is a substrate?

The molecule an enzyme acts on

What is the active site?

Region where substrate binds on enzyme

What is an enzyme-substrate complex?

Temporary complex formed when enzyme binds substrate

What happens during enzyme action?

Substrate binds → reaction occurs → products released

What is the lock and key model?

Substrate fits exactly into enzyme active site (rigid model)

Why is lock and key model limited?

Enzymes are flexible and can change shape

What is the induced fit model?

Enzyme changes shape to fit substrate better

How do enzymes lower activation energy?

lower activation energy by binding substrates in ways that make reactions easier to occur. They can orient substrates correctly for collision, strain or weaken existing bonds, create a favourable chemical environment, and form temporary interactions with substrates that stabilise the transition state and promote the reaction.

Do enzymes change ΔG?

No, they only lower activation energy

What factors affect enzyme activity?

pH; temperature; environment; cofactors

What are cofactors?

Inorganic ions required for enzyme function

What are coenzymes?

Organic molecules that assist enzymes

What are competitive inhibitors?

Bind active site; compete with substrate

What are non-competitive inhibitors?

Bind elsewhere; change enzyme shape

What is allosteric regulation?

Binding at regulatory site affects enzyme activity

What are allosteric activators?

Stabilise active form of enzyme

What is feedback inhibition?

End product inhibits early enzyme in pathway

What is protease activation?

Enzymes produced inactive and activated when needed

What is cellular respiration?

Process that converts glucose + O2 → CO2 + H2O + ATP

What type of reaction is respiration?

Redox (electron transfer)

What is oxidation?

Loss of electrons

What is reduction?

Gain of electrons

Where does respiration occur?

Cytosol and mitochondria

What is the structure of mitochondria?

Outer membrane; inner membrane; cristae; matrix; intermembrane space

What is the outer membrane?

Smooth outer boundary

What is the inner membrane?

Folded membrane containing ETC

What are cristae?

Folds increasing surface area

What is the matrix?

Inner space where Krebs cycle occurs

What is intermembrane space?

Space between membranes

What are the 3 stages of respiration?

Glycolysis; Krebs cycle; electron transport chain

Where does glycolysis occur?

Cytosol

Where does Krebs cycle occur?

Matrix

Where does ETC occur?

Inner membrane

What happens in glycolysis?

Glucose → 2 pyruvate; 10 enzyme steps

What is energy investment phase?

Uses 2 ATP

What is energy payoff phase?

Produces 4 ATP and 2 NADH

What is net ATP from glycolysis?

2 ATP

What is NADH?

Electron carrier

What happens to pyruvate?

The pyruvate moves into the matrix of the mitochondria and is converted into Acetyl CoA which enters the Citric Acid cycle

CO2 released

What happens in Krebs cycle?

Acetyl-CoA + oxaloacetate → citrate → CO2 + NADH + FADH2 + ATP

What is regenerated in Krebs cycle?

Oxaloacetate

What is produced in Krebs cycle?

6 NADH; 2 FADH2; 2 ATP; 4 CO2

the NADH from glycolysis and the NADH and FADH2 from the Citric Acid cycle enter

an electron transport chain in the inner membrane of the mitochondria.

What happens in electron transport chain?

Electrons passed through protein complexes releasing energy

What is the role of oxygen?

Final electron acceptor

What happens to H+?

Pumped into intermembrane space

What is chemiosmosis?

H+ flows through ATP synthase to generate ATP

What is ATP synthase?

Enzyme that produces ATP

What is oxidative phosphorylation?

ATP production via ETC and proton gradient

How many ATP per glucose?

~36–38 ATP

What is fermentation?

Process used when oxygen is absent

Why is fermentation needed?

Regenerates NAD+ so glycolysis can continue

What are the types of fermentation?

Lactic acid fermentation; alcohol fermentation

What happens in lactic acid fermentation?

Pyruvate → lactate

What happens in alcohol fermentation?

Pyruvate → ethanol + CO2

Metabolism definition

Metabolism is the totality of an organism's chemical reactions — the sum of all chemical processes that occur in a living cell or organism to maintain life. It includes both catabolic (breaking down) and anabolic (building up) pathways.

Catabolic vs anabolic pathways

ATP reaction

ATP + H₂O → ADP + Pᵢ + Energy (~30.5 kJ/mol)

why energy coupling is important

• Allows cells to carry out non-spontaneous reactions (building macromolecules, active transport, muscle contraction) that are essential for life

• ATP is the universal coupling agent in cells

• Makes metabolism efficient — energy from catabolic reactions isn't wasted; it's captured in ATP and reused

atp cycle

whats a kj

A kilojoule (kJ) is a unit used to measure energy

whats a mole

a unit amount of a substance. 1 mole = 6.02 × 10²³ particles