Activity 7 physio lab

The ________- technique is a qualitative method suitable for the isolation and identification of heavier eggs such as trematodes or flukes (e.g. Paragonimus spp.)

fecal Sedimentation

Most trematodes are too large and heavy to float reliably in the flotation fluids that is usually  used for nematode eggs, They do sink rapidly to the bottom of the fecal/water suspension. True or False

True

What stain do we use in fecal sedimentation technique?

Methylene blue

Examination of the fecal material as a direct smear may or may not reveal organisms. It may not be sensitive enough to detect low parasite loads or certain parasitic stages. True or False

True

Microscopic examination of the feces is essential to detect the presence of intestinal parasites and to recognize and identify them. It is also vital to increase the probability of finding parasites in fecal samples for an accurate diagnosis. True or False

True

________ is a good technique to rapidly identify the presence of intestinal parasites, including motile trophozoites, cysts, eggs, or larvae in fresh stool samples.

Direct fecal examination

What solution do we use in direct fecal examination

Saline solution

What stain do we use in direct fecal examination?

Ziehl-Neelsen, carbol-fuchsin and methylene blue

________ is by far the most common diagnostic procedure used to detect intestinal parasites in our veterinary species due to its low cost and is a relatively straightforward procedure to conduct.

Fecal flotation

The majority of parasite ova/ eggs have a specific gravity (SG) that falls between _________, thus the fecal flotation relies on the ascension of eggs and ova using the difference in overall density to the flotation solution. 

1.05 and 1.23

The most common flotation solutions used in veterinary medicine include _____________.

Saturated sodium chloride, sodium nitrate, sugar (e.g.  Sheather’s solution), magnesium sulfate, and zinc sulfate

For example, __________ have an SPG of 1.25 resulting in Giardia cysts becoming distorted and difficult to identify for the novice, but a better overall ova yield than other solutions.

sugar solutions

What rpm do we use in Sheather’s Sugar Solution Technique for fecal floating technique?

2000 rpm