Laboratory 6: Intro to Genetic Engineering: Bacterial Transformation

These flashcards cover key concepts and terms related to genetic engineering and bacterial transformation from the lecture notes.

By genetic engineering, we mean artificially and directly changing the __ of an organism.

DNA

The process of taking up plasmids by bacterial cells is called __.

transformation

The plasmid DNA we will use today is called __.

pGLO

The gene __ confers antibiotic resistance, allowing the bacterium to survive on media with ampicillin.

bla

The gene __ codes for Green Fluorescent Protein, which glows under UV light when arabinose is present.

gfp

__ techniques are important to avoid contamination when handling E. coli culture.

Aseptic

Negative controls ensure that there is __ when there should be no effect.

no effect

Positive controls ensure there is an __ when there should be an effect.

effect

E. coli has a __ chromosome that encodes its basic genes and is relatively simple to genetically engineer.

circular

The __ in the CRISPR/Cas9 system is a series of repeats that allows protection against viruses.

CRISPR