Barrett Quiz

How to get the Amino acid on t RNA

• Aminoacyl tRNA synthetase, enzyme

• ATP comes into position

• AA comes into opp

• Amino acid + ATP +> throw off a pyrophosphate and create AA-AMP (Animoaceyl AMP)

• tRNA d-loop side goes into it’s position

• Reaction with the carboxylic acid end of the AA allows to be bound to -OH acceptor stem of tRNA => charged tRNA (with an AA)

• Protein elongation factors escort the charged tRNA to the Ribosome

Initiation with Eukaryotes

1) Assembly of the factors for the 43s complex on the small subunit

2)  Attachment of mRNA to 43s complex

3) Scan the mRNA for the start codon -AUG methianine

4) Attachment of the 60s Large subunit to form a complete ribosome (mRNA running through the middle)

Elongation with Eukaryotes

1) Peptide bond formation

2) Translocation

Termination with Eukaryotes

1) Release factor (RF) III, I, II + Ribsome recycling factor  (RRF)=> disassemble the ribosome

2) Peptide released & goes to do wtv

info.

• 9-10 AA: identifer, signal sequence

• SRP takes the polypeptide to the plasma mem of the Rough ER

• SRP is going to recognize the polypeptide => takes to signal recognition particle receptors & docs

• Hydrolysed GTP causes opening of Translocon

• Polypeptide goes in lumen

• Signal peptidase reads signal through => signal to cleave the polypeptide

• Sent to Golgi => modified and packaged within a secretory vesicle