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25 Terms
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Artefacts
Objects or structures seen through a microscope that have been created during th processing of the specimen.
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Cell wall
A strong but flexible layer that surrounds some cell-types.
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Centrioles
Component of the cytoskeleton of most eukaryotic cells, composed of microtubules.
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Chloroplasts
Organelles that are responsible for photosynthesis in plant cells. Contain chlorophyll pigments, which are the site of the light reactions of photosynthesis.
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Contrast
Staining or treating specific cell components so they are visible compared to untreated components.
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Counterstain
Application of second stain with a contrasting colour to sample for microscopy.
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Cytoplasm
Internal fluid of cells, composed of cytosol (water, salts and organic molecules), organelles and cytoskeleton.
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Cytoskeleton
A network of fibres in the cytoplasm of a eukaryotic cell.
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Differential staining
Using specific stains to distinguish different types of cell.
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Electron microscopy
Microscopy using a microscope that employs a beam of electrons to illuminate the specimen. As electrons have a much smaller wavelength than light they produce images with higher resolutions than light microscopes.
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Eukaryotic cells
Cells with a nucleus and other membrane-bound organelles.
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Golgi apparatus
Organelle in most eukaryotic cells formed from an interconnected network of flattened, membrane-enclosed sacs, or cisternae. Play a role in modifying and packaging proteins into vesicles.
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Laser scanning confocal microscope
A microscope that employs a beam of fluorescence and a pin-hole aperture to produce an image with a very high resolution.
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Light microscope
An instrument that uses visible light and glass lenses to enable the user to see objects magnified many times.
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Organelle
Membrane-bound compartments with varying functions inside eukaryotic cells.
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Prokaryotic cells
Cells with no membrane-bound nucleus or organelles.
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Resolution
The shortest distance between two objects that are still seen as separate objects.
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Scanning electron microscopy
An electron microscope in which a beam of electrons is sent across the surface of a specimen and the reflected electrons are focused to produce a three- dimensional image of the specimen surface.
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Scanning electron microscopy (SEM)
An electron microscope in which a beam of electrons is sent across the surface of a specimen and the reflected electrons are focused to produce a threedimensional image of the specimen surface.
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Smooth endoplasmic reticulum
Endoplasmic reticulum lacking ribosomes; the site of lipid and carbohydrate synthesis, and storage.
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Stage graticule
A slide with a scale in micrometres (μm) etched into it. Used to measure the size of a sample under a light microscope.
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Stains (staining)
Dyes used in microscopy sample preparation to increase contrast or identify specific components.
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Transmission electron microscopy (TEM)
An electron microscope in which a beam of electrons is transmitted through a specimen and focused to produce an image.
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Ultrastructure
Fine internal cellular details that can be resolved using an electron microscope.
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Vacuoles
Membranous sacs used to transport materials in the cell.