Intro

Whole blood contains

All cellular formations, antibodies, and clotting factors

Packed red cells contain

Only RBCs

Fresh frozen plasma contains

Antibodies and clotting factors

Platelet units contain

Minimal amounts of RBCs, antibodies, clotting factors, and platelets

Cryoprecipitate contains

Clotting factors only

ABO whole blood compatibility

Type specific only

Packed cell compatibility

O to everything, A and B to AB, O gets nothing

Plasma product availability

AB to everything, A and B to AB, AB receives nothing

Blood type makes antibodies against

The type they don’t like

Antibodies in plasma

A: anti-B

B: Anti-A

AB: none

O: anti-a and anti-b

Antigens on red blood cell

a: a

b: b

AB: a and b

O: none

Blood bank functions

Facility that collects, stores, processes, and distribution for blood products for transfusion

Regulatory orgs for blood banks

FDA, CMS, State health depts, and OSHA

Accreditation agencies for blood banks

AABB, JCO, CAP, FACT, NMDP, and WMDA

Regulations under FD and C act, and PHS

Blood and its components are drugs, obtain bio licenses to ship in interstate commerce, mandate adherence to cGMP, follows unit from collection to transfusion

FDA regulations:

CBER: inspections, medical devices used

ORA: responsibility over all field operations, inspections, and investigations of blood and device manufacturers

FDA inspections

Pre-license/ approval

Routine inspection

“For cause” (serious injury)

Class I recalls

Reasonable probability of serious/ fatal effects

Class II recall

May cause temporary adverse effects or remotely serious problems

Class III recalls

Not likely to cause adverse consequences (most common)

AABB checklist updated

Every 2 years

AABB and CAP accredited labs have physical inspections every

2 years

Main difference between blood bank w a collection facility and those without 🤨

Ones w /collection units collect their own supply of blood units

Functions of a donor center

Recruitment, prep and storage, processing (testing) , labeling, HIV database, paternity testing, and rare donor recruitment

Function of a reference blood bank lab

Problem solving: ABO discrepancies, RH discrepancies, antibody ID, molecular genotyping, DAT, warm and cold autoantibodies, transfusion reaction workup

Positive DAT indicates:

Immune reaction to drug or disease

Current or delayed immunocompatibility

Warm and cold autoantibodies

Cause positive DAT and can mask alloantibodies

Transfusion reaction workup

Investigate cause of symptoms following a transfusion reaction