Complete Guide to Phlebotomy: Order of Draw, Tube Additives, and Specimen Handling
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50 Terms
What is the correct order of draw?
Yellow → Light Blue → Red → Red/Gray MARBLE → Green → Green/Gray MARBLE → Lavender → Gray
Which tube is drawn first?
Yellow
Which tube is drawn last?
Gray
Where is Red/Gray MARBLE in the order?
After Red (4th position)
Which tube comes after Red?
Red/Gray MARBLE
Which tube comes before Lavender?
Green/Gray MARBLE
Why must order of draw be followed?
To prevent additive contamination
What happens if additives mix between tubes?
Inaccurate lab results
Which tube is used for cultures?
Yellow
Which tube is used for coagulation testing?
Light Blue
What additive is in Yellow tubes?
Sodium polyanethol sulfonate
What does sodium polyanethol sulfonate do?
Prevents clotting and stabilizes bacterial growth
What additive is in Light Blue?
Sodium citrate
What does sodium citrate remove?
Calcium
What additive is in Green?
Heparin
What does heparin inhibit?
Thrombin formation
What additive is in Lavender?
EDTA
What does EDTA remove?
Calcium
What additives are in Gray?
Potassium oxalate and sodium fluoride
What does sodium fluoride inhibit?
Glycolysis
What is Yellow used for?
Blood or body fluid cultures
What is Light Blue used for?
Coagulation testing
What is Red used for?
Serum, chemistry, blood bank, immunology
What is Red/Gray MARBLE used for?
Serum, chemistry, immunology
What is Green used for?
Chemistry testing
What is Green/Gray MARBLE used for?
Plasma determinations in chemistry
What is Lavender used for?
Hematology
What is Gray used for?
Glucose and alcohol testing
Which tube is used for hematology?
Lavender
Which tube is used for glucose testing?
Gray
How many inversions are needed for Light Blue?
3-4
How many inversions are needed for Red?
5
How many inversions are needed for Red/Gray MARBLE?
5
How many inversions are needed for Green?
8
How many inversions are needed for Green/Gray MARBLE?
8
How many inversions are needed for Lavender?
8
How many inversions are needed for Gray?
8-10
Why must tubes be inverted?
To properly mix additives and prevent errors
What does a centrifuge do?
Separates blood components by weight
Where do heavier components go in a centrifuge?
Bottom
Where do lighter components go in a centrifuge?
Top
Why must serum clot before centrifuge?
To allow proper separation
What is aliquoting?
Transferring specimen into other tubes
Why is pouring blood not allowed?
Causes contamination and inaccurate results
Where are specimens placed after collection?
Biohazard bag
Why is temperature important for specimens?
It affects accuracy of results
How are light-sensitive specimens protected?
Foil or amber tube
Who reviews lab results first?
Provider
What are critical lab values?
Life-threatening abnormal results
What must be done immediately for critical results?
Report to provider immediately