Evacuated Tubes -- Everything and Anything

What additives are in the light blue tube?

Sodium Citrate

What is the function of sodium citrate in the light blue tube?

Sodium citrate acts as an anticoagulant, preventing blood clotting by binding calcium ions in the sample.

What section of the lab does the light blue tube go into?

Coagulation

How many inversions does the light blue tube require?

3-4 inversions

what is the sample type for the light blue tube?

plasma

because sodium citrate prevents clotting, you get _____ after centrifugation

plasma

what tests require the light blue tube?

PT, APTT, PTT, Fibrinogen, D-Dimers

What is the ration of blood to sodium citrate?

9:1

what are the requirements for the light blue tube?

it must be filled to draw capacity and all vacuum must be exhausted

what are the additives in the royal blue tube with LAVENDER stripe?

sodium edta

how many times do you invert the royal blue tube with lavender stripe?

8-10 times

what tests require the royal blue with lavender stripe?

toxicology, nutritional chemistry, and tests for elements like lead, arsenic, mercury, zinc, and aluminum

what section of the lab should the royal blue tube with lavender go to?

chemistry/trace elements

what sample type is the royal blue tube with lavender stripe?

whole blood or plasma

what does SODIUM EDTA in the ROYAL BLUE with LAVENDER STRIPE do?

it inactivates thrombin and thromboplastin

ok bestie, what additive(s) are in the orange tube

thrombin

what does thrombin in the orange tube do?

it accelerates clotting

how many inversions for the orange tube

5 inversions

what sample type is the orange tube

serum

what section of the lab does the orange tube go to?

STAT testing

what tests are the orange tube used for

serum chemistries

what additives are in the grey tube

potassium oxalate and sodium fluoride

what is the purpose of potassium oxalate?

stops coagulation process

how exactly does potassium oxalate stop the coagulation process?

by binding with calcium

what is the purpose of sodium fluoride?

it acts as an antiglycolytic agent (glucose preservative — gray loves glucose) by preventing red blood cells from using glucose and changing it into lactic acid

what is the antiglycolytic agent in the gray tube called?

glycolytic inhibitor

what section of the lab does the gray tube go to

chemistry

how many inversions for the gray tube

8-10 inversions

what tests are conducted using the gray tube?

FBS (fast blood sugar), Lactic Acid, Blood Alcohol Concentration (BAC)

what sample type is the gray tube

plasma

what additives are in the pink tube

EDTA spray

how many inversions for the pink tube

8-10 inversions

what section of the lab does the pink tube go to

immunohematology

what tests are in the pink tube?

blood typing, donor blood testing, cross-matching blood, preparation of blood and blood products for transfusion

what is the blood sample type for pink tube

whole blood

what is the function of the yellow tube with acid citrate dextrose?

it maintains red cell viability or growth

what additives can be added to the yellow tube?

acid citrate dextrose and sodium polyanethol sulfonate

how many inversions for the yellow tube with ACD

8-10 inversions immediately

what tests are conducted using the yellow tube with ACD

cellular studies, human leukocyte antigen typing (HLA)

what section of the lab does the yellow tube with ACD go in?

blood bank, HLA lab

what sample type is the yellow tube with acd

whole blood/plasma

what is the functions of sodium polyanethol sulfonate in the yellow tube

it acts as an anticoagulant and neutralizes the effect of bacterial growth inhibitor such as antibiotics

what is the lab section for the yellow tube with SPS

microbiology and blood cultures

what is the sample type for the yellow tube with SPS

whole blood

how many inversions for the yellow tube with SPS

8-10 times immediately

what tests are for the yellow tube with sps?

blood cultures to detect bacterial or fungal infections in the blood stream

where in the order of draw does the yellow tube with sps go?

the tube is sterile so it has to be done before other tests but when blood culture bottles are ordered with coagulation tests, this tube acts as a discard tube