Microbiology: culturing and media
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38 Terms
Nutrient
media that contains generic nutrients to enable the growth of most nonfastidious organisms
Enriched
media that contains complex substances for the needs of a specific fastidious species
Selective
media that inhibits the growth of selected organisms and allows the growth of others
Differential
media that can grow several different organisms that demonstrate different visual characteristics
Enrichment
media type that is designed to encourage the growth of small numbers of a particular organisms while suppressing the growth of others
Broth
media type that is used in combination with growth on agar plates and used to detect small numbers of most aerobes, anaerobes, and microaerophiles
Transport
media type that is designed to maintain the viability of organisms without allowing them to multiply
nutrient
What kind of media is sheep blood agar (SBA)?
nutrient, enriched, differential
What kind of media is blood agar (BA)?
enriched
What kind of media is chocolate agar?
selective, differential
What kind of media is MacConkey agar (MAC)?
selective
What kind of media is phenylethyl alcohol agar (PEA)?
selective
What kind of media is Columbia colistin nalidixic acid agar (CNA)?
selective
What kind of media is mannitol salt agar (MSA)?
enrichment, broth
What kind of media is LIM?
broth
What kind of media is thioglycolate (THIO)?
Cary-Blair, Amie’s, Stuart’s, viral
What are some kinds of transport media?
Inhibits growth of gram+ bacteria, promotes growth of gram-.
Differentiates organisms on the basis of lactose fermentation: lactose-fermenting will form a pink colony, non-lactose-fermenting will form clear colonies.
What is the function of MAC agar?
Inhibits growth of gram- bacteria, promotes growth of gram+
What is the function of PEA or CNA agar?
Inhibits most organisms except Staphylococcus
What is the function of MSA agar?
Differentiates organisms based on hemolysis
What is the function of BA agar?
organism that requires oxygen to grow
What is an obligate aerobe?
organism that requires reduced oxygen and increased CO2 to grow
What is a microaerophile?
organism that requires no oxygen present to grow
What is an obligate anaerobe?
organism that grows both with and without oxygen; usually tends to grow better with oxygen; majority of bacterial pathogens
What is a facultative anaerobe?
organism that requires increased CO2 to grow
What is a capnophilic organism?
organism that grows at 10-20°C; few pathogens
What is a psychrophile?
organism that grows at 20-45°C; human pathogens and most bacteria
What is a mesophile?
organism that grows over 50°C; few pathogens
What is a thermophile?
35°C
At what temperature do most organisms grow?
6.5-7.5 pH
What pH is media buffered to?
70-80%
At what % humidity are organisms usually incubated at?
48-72 hours
How long are most organisms incubated for?
5-7 days
How long are anaerobic cultures incubated for?
Utilizes room air, usually at 35°C, high humidity
What are the conditions of an ambient air incubator?
Utilizes 5-10% CO2 mixed with room air, usually at 35°C, high humidity
What are the conditions of a CO2 incubator?
Utilizes 5% hydrogen, 5-10% CO2, and 85-90% N2, usually at 35°C, high humidity
What are the conditions of an anaerobic incubator?
2 years
How long are documentation/records kept for reagents, stains, and media?