109C quiz 3

Edman reagent for?

find N term AA

carboxypeptidases for? A vs B?

Find C term AA, A is not for Arg or Lys, B is only for those AA

trypsin

cleaves bond on C side of Arg and Lys

Chymotrypsin

cleaves bond on C side of Phe, Tyr, Trp

Elastase

cleaves on C side of Val, Ala, Gly, Ser (VAGS r elastic)

Cyanogen Bromide

hydrolyze peptide bond on C side of Met

NAD+/NADP+

NADH/NADPH

FAD

TPP-ylide

Biotin

PLP

THF

ATP

THF also called

folic acid

NAD/NADP also called

niacin, B3

TPP also called

thiamine B1

FAD also called

riboflavin, B2

Biotin also called

vitamin H, B1

PLP also called

pyridoxine, B6

NAD/NADPH used for

oxidation/reduction w czrboxyl

FAD used to

oxidation into double bonds

TPP used to

acyl group transfer

Biotin used for

carboxylation

PLP used for

decarboxylation (removing CO2)

THF used for

carbon transfer

How biotin is used w Mg and ATP

Mg binds w ATP—> ATP activates bicarbonate —> reacts w biotin