3.1 DNA Replication, RNA Transcription

The enzymes in DNA replication are:

• ____

• ____ ____

• ____ ____

• ____ ____

The enzymes in DNA replication are:

• Helicase

• RNA Primase

• DNA Polymerase

• DNA Ligase

• Helicase unwinds DNA

• RNA Primase lays down a primer on _ end with __ RNA nucleotides and acts as starting point for duplication

• DNA Polymerase:

  • Finds primer and starts ____ ____

  • Replaces ____ ____ ____ with ____ ____

  • ____ DNA

• Ligas binds nucleotides with ____ bonds.

• Helicase unwinds DNA

• RNA Primase lays down a primer on 3` end with ~20 RNA nucleotides and acts as starting point for duplication

• DNA Polymerase:

  • Finds primer and starts pairing nucleotides

  • Replaces primase RNA nucleotides with DNA nucleotides

  • Proofreads DNA

• Ligas binds nucleotides with covalent bonds.

In duplication, DNA polymerase reads from __ to __ and builds from __ to __.

The __ to strand is called the ____ strand; the other is called the ____ strand.

In duplication, DNA polymerase reads from 3` to 5` and builds from 5` to 3`

The 3` to 5` strand is called the leading strand; the other is called the lagging strand.

When duplicating on the lagging strand, each piece is called an ____ ____.

When duplicating on the lagging strand, each piece is called an okazaki fragment.