PARA LAB 1 F

SCIENTIFIC NAME

BEST DIAGNOSTIC TEST

SPECIMEN

INFECTIVE STAGE

STAIN

SN: Entamoeba histolytica cyst

DT: ELISA

SPECIMENS

• Freshly passed stool

• Sigmoidoscopy Materials

• Hepatic abscess material

IS: Quadrinucleated cyst

STAIN

Lugol’s iodine (cyst)

Quensel’s Methylene Blue (trophozoite)

SCIENTIFIC NAME

Entamoeba histolytica trophozoite

TREATMENT

Metronidazole or Tinidazole

SCIENTIFIC NAME

Entamoeba coli cyst

SCIENTIFIC NAME

Entamoeba coli trophozoite

Small race of E. histolytica

Entamoeba hartmanni

SCIENTIFIC NAME

Entamoeba hartmanni cyst

SCIENTIFIC NAME

Entamoeba hartmanni trophozoite

SCIENTIFIC NAME

Endolimax nana cyst

SCIENTIFIC NAME

Endolimax nana trophozoite

SCIENTIFIC NAME

Iodamoeba butschlii cyst

SCIENTIFIC NAME

Iodamoeba butschlii trophozoite

SCIENTIFIC NAME

HABITAT

MOT

LABORATORY DIAGNOSIS

Entamoeba gingivalis trophozoite

Oral Cavity

Oral Contact

Mouth scraping

SCIENTIFIC NAME

Naegleria fowleri cyst

SCIENTIFIC NAME

Naegleria fowleri trophozoite

SCIENTIFIC NAME

Naegleria fowleri flagellate

Naegleria fowleri INFECTIVE STAGE

Ameboid trophozoite

Naegleria fowleri SPECIMEN

CSF

Tissue samples

Nasal discharges

Naegleria fowleri DIAGNOSTIC TEST

Microscopic examination

● Complete Blood Count

● Culture:

- Bacteria Seeded Agar

- Immunohistochemical staining

- Immunofluorescent antibody (IFA)

- PCR

- CSF Analysis

Naegleria fowleri DIAGNOSTIC STAGE

Ameboid trophozoite

Flagellated trophozoite (CSF)

SCIENTIFIC NAME

Acanthamoeba spp. cyst

SCIENTIFIC NAME

Acanthamoeba spp. trophozoite

Acanthamoeba spp. INFECTIVE STAGE/FORM

trophozoite

Most common Acanthamoeba spp.

Acanthamoeba castellani

Acnthamoeba spp. CAUSES

• Granulomatous Amebic Encephalitis (GAE)

• Acanthamoeba keratitis

INFECTION CAUSED by Naegleria fowleri

Primary Amebic Meningoecephalitis

Naegleria fowleri SAMPLE

CSF

Brain-eating amoeba

Naegleria fowleri

CONCENTRATION TECHNIQUES

Sedimentation Technique

Floatation Technique

All purpose fixative

10% Formalin

Dissolve neutral fats in the stool

Ether

-Alternative for ether

-More efficient in the recovery of cestode eggs and Giardia cysts.

Ethyl acetate

SEDIMENTATION TECHNIQUE REAGENTS

• 10% Formalin

• Ether

• Ethyl acetate

Reagent used if 10% formalin is not available

5% Formol Saline

LAYERS

LAYERS

SPECIFIC GRAVITY OF FRESH STOOL SAMPLE

1.18 specific gravity

SPECIFIC GRAVITY OF FORMALIN-FIXED SAMPLES

1.20 specific gravity

Most common reagents in CENTRIFUGAL FLOATATION TECHNIQUE and their SPECIFIC GRAVITY

ZnSO4 - Zinc sulfate (1.18)

NaCl - Sodium Chloride (1.2)

Sheather’s (1.27)

Sheater’s Preservative used

Phenol Formalin

Sheather’s sugar is used for

Coccidian oocyst and isospora

FILL IN THE BLANK