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what happens when milk goes off (RP)
bacteria in it breaks down lactose into lactic acid, decreasing pH
role of lipase in milk RP
speeds decay up and breaks down lipids in milk to produce 3 fatty acids, and glycerol. The fatty acids decrease the pH and make it acidic
indicator of milk RP
phenolphthalein is pink at alkali and colorless at acid
why is sodium hydrocarbonate added to milk for the decay RP
to make it alkali so phenolphthalein works
milk RP basic method
milk alkali solution is heated up and lipase is added. it is stirred continuously while a stopwatch goes until it goes colourless (white) and acidic
milk decay RP resuts
rate of decay increases with temperature until a temperature where it sharply drops due to denaturing