L23 Light Microscopy
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20 Terms
4 Properties of light relevant to light microscopy
Ways light-matter interactions can change the properties of light
Basic Structure of a Transmission, Bright Field Light Microscope
Contrast definition
Resolution definition
Angular resolution
The Rayleigh Criterion formula for angular resolution
Spatial resolution calculation
How to improve the resolution
The Point Spread Function
The two types of ligth microscopy
Common Transmission Microscopy Modes
Anatomy of a Wide-Field Fluorescence Microscope
Use of changing the filters in fluorescence microscopy
The Point Spread Function in Wide-Field Fluorescence Microscopy
Selective imaging of sites using fluorescence microscopy
Confocal microscopy
Super-resolution microscopy: SIngle-Molecule Localisation Microscopy
Stimulated emission depletion (STED)
Photoactivated Localization Microscopy (PALM)
