[august mtle 2025] blood banking rbc preservation techniques

rbc viability

the goal of blood banking is to maintain?

>75%

____ of transfused RBCs should remain viable or still functional in the bloodstream for at least 24 hours

24 hours

75% of transfused RBCs should remain viable in the bloodstream for at least how many hours?

1%

free hemoglobin should be less than ____ of total hemoglobin

1-6 degrees Celsius

the storage temperature requirement of liquid whole blood and RBC units

1-10 degrees Celsius

shipping or transport temperature of liquid whole blood

ice packs

what should be included in the shipping or transport of liquid whole blood?

4 hours

the temperature of the storage of liquid whole blood and RBC units should be done every?

plasma potassium

plasma hemoglobin

lactic acid

which parameters in the RBC will increase due to storage lesions?

pH

2,3-DPG

glucose

ATP

which parameters in the RBC will decrease due to storage lesions

rbc storage lesions

biochemical changes seen in stored whole blood

lactic acid

this increases due to extensive glycolysis

pH

this decreases due to the increase in lactic acid

plasma potassium

plasma hemoglobin

these increase due to hemolysis

approved preservative solutions

these are present in primary blood bags

21 days

shelf life of acid-citrate-dextrose formula A

21 days

shelf life of citrate-phosphate-dextrose

31 days

shelf life of citrate-phosphate-dextrose-adenine

adenine

this component maintains ATP levels in CPDA-1

21 days

shelf life of citrate-phosphate-double dextrose

acid-citrate-dextrose Formula A

this preservative solution has a lower pH and has a loss of 2,3 DPG in the first week

citrate-phosphate-dextrose

this preservative solution contains phosphate

phosphate

this component of CPD preserves 2,3 DPG

citrate-phosphate-double dextrose

this preservative solution has 100% more glucose than CPD

additive solutions

these solutions are usually added to packed rbcs

42 days

additive solutions can extend the shelf life for up to?

72

additive solutions are added to rbcs within the first ____ hours of storage

saline

adenine

glucose

mannitol

components of additive solutions

saline

this component of additive solutions maintains the isotonic state of the rbcs

adenine

this component of additive solutions maintains ATP levels

glucose

this component of additive solutions maintains the energy present

mannitol

this component of additive solutions prevents hemolysis

AS-1

code for adsol

AS-3

code for nutricell

AS-5

code for optisol

AS-7

code for SOLX

rejuvenation solution

this solution provides an extension of 24 hours

3 days

rejuvenation solutions can be used up to ____ after the expiration of red cells

phosphate

inosine

pyruvate

adenine

contents of rejuvenation solution

phosphate

this component of rejuvenation solution serves as the buffer

inosine

pyruvate

these components of rejuvenation solution maintains 2,3 DPG

adenine

this component of rejuvenation solution maintains ATP levels

rejuvesol

this is the FDA-approved rejuvenation solution in the US

red cell freezing

this extends the shelf-life of red cells for up to 10 years

-65 degrees Celsius

this is the maintaining temperature of red cell freezing

red cell freezing

this method is usually for autologous units and includes an addition of a cryoprotective agent to rbcs

6 days old

red cell freezing includes the addition of a cryoprotective agent to RBCs that are less than how many days old?

glycerol

the most common cryoprotective agent for red cell freezing is?

40% w/v

20% w/v

what are the two common concentrations of glycerol for red cell freezing?

glycerol

this agent prevents intracellular dehydration of RBCs while in storage

deglycerolization

when using frozen red cells, this is done prior to transfusion

washing

deglycerolization is done by?

NSS

what is usually used for deglycerolization?