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Gene
Part of the DNA that codes for proteins and RNA (tRNA and mRNA)
Introns
98.5% - non-coding genes
1.5%
percentage of coding genes or Exons
20,000 to 25,000
how many genes in the human genome codes for an average of three proteins.
GENE POOL
Total genetic diversity found within a population of a species
DNA FINGERPRINTING
Method used to identify an individual from a sample of DNA by looking at unique patterns in their DNA.
DNA FINGERPRINTING
A process or technique of analysis revealing unique patters of an individual’s DNA involving non-coding regions
1980
in this year, American researchers discover non-coding regions of DNA
1987
In this year, the First conviction of man in England based on DNA evidence
Professor Alec Jeffreys
In 1987, This professor, developed the process of the DNA fingerprinting
3 billion
Human genome - contains how many nucleotides
Minisatellite
also known as Variable Number of Tandem Repeats
Minisatellite
10-60 base pairs
Microsatellite
2-6 base pairs
Microsatellite
Simple Sequencing Repeats (SSRs) or Short Tandem Repeats (STRs)
Variable Number of Tandem Repeats
Repetitive sequence that are highly variable
tetranucleotide
Short tandem repeats: AAAG AAAG AAAG AAAG
(tetranucleotide, trinucleotide, dinucleotide)
Homozygote
both alleles are the same length
Heterozygote
alleles differ and can be resolved from one another
ELECTROPHORESIS
mobility/direction of movement of a certain electrical point.
1. Cells broken down to release DNA
2. DNA strands cut into fragments
3. Fragments separated (Gel electrophoresis)
4. Transfer bands to nitrocellulose
5. Probing
6. Pattern of fragments analysed
steps involved in DNA fingerprinting
DNA Extraction
Steps/ how to get the genome of interest. Used in paternity testing, crime scene (usually).
DNA Cutting
The DNA is cut up into fragments using restriction enzymes
Sticky ends
Single Stranded ends of the restriction fragment
blunt ends
Straight ends w/o any single strand region
Fragment Separation
Steps: Samples containing the fragments are placed into individual wells in a gel using a pipette
Paternity disputes
Criminal Investigation and Forensic Science
. Genetic Screening and Developing Cures
Screening Test
Developing Cures for Inherited Disorders
Genetic Archaeology
application of DNA fingerprinting
Tetranucleotides
favored in human identity
14
aaga is a particular individual with how many repeats?
false - heterozygote
homozygote is alleles differ and can be resolved from one another. (true/false)
Restriction fragment length polymorphism (RLFP)
enzymatic procedure for separation and identification of desired fragments of DNA
fragment separation
samples containing the fragments are placed into individual wells in a gel using pipette
agarose gel electrophoresis
different banding patterns from different individualls
dna dutting
the dna is cut up into fragments using restriction enzymes
1983
Narborough village murders
year when first reported use of genetic fingerprinting in a criminal case and what is the crime
225 000 lost
800 victims identified
how many lives was lost during soutasian tsunami and within 3 months how many victims have been identified