Chapter 6 Lumio/Mentimeter

What is the purpose of M/Sorb agar?

Grows GNRs, but specifically for growth of E. coli 0157:H7

What do pink colonies on a M/Sorb plate indicate?  

Sorbital was fermented so the organism isn’t E. coli 0157:H7

What is the purpose of SBA agar?

Grows GPC and GNR, but won’t grow Haemophilus

What is the purpose of GN Broth?

Grows GNRs ONLY

What is the purpose of LIM Broth?

Aids in growth of group B strep

Which media is capable of showing lactose and non-lactose fermentation?

MAC

What is the purpose of THIO Broth?

Grows all bacteria including anaerobes

In order for THIO broth to work correctly, what must be done to the media?

Oxygen must be removed

Neisseria organsims will grow on which media types?

CHOC, TM, and least used SBA

What technique is used when streaking plates for a urine culture?

Quantitative Isolation Streak

Processes done to sputum and bronchial washings before setup:

Decontamination and Digestion

What stool pathogen will appear as clear colonies on an XLD or HE plate?

Shigella

What is the purpose of EMB agar?

Grows all GNRs

What is the most important step in performing a urine culture?

Mix the specimen before placing urine on media

What is the purpose of MAC agar?

Grows all GNRs

What is the term used to describe partial lysis of RBCs around individual bacteria giving media a greenish appearance?

Alpha

What is the purpose of Selenite Broth?

Aids in grows of GRNs (specifically Salmonella/Shigella)

How should a sputum specimen be stored until it is inoculated onto media?

Refrigerator

What is the term used to describe complete lysis of RBCs around each colony?

Beta

Viral media must inhibit growth of what?

Bacteria and Fungus

What is the greatest variable in blood culture collection?

How much blood is collected

What do clear colonies on a M/Sorb plate indicate?

Sorbital was not fermented, could possible be E. Coli

Colonies on a MAC plate which are non-lactose fermenters will have what appearance?

Clear

A urine for culture is received in a tube preservative. How should it be stored before setup?

Room Temperature

HE media is only used when inoculating what culture type?

Stool

What media are capable of showing H2S production?

XLD and HE

A urine streaked with a 0.001 loop has 56 colonies. What is the colony count?

56,000

What is different about specimen containers in which anaerobic cultures are collected?

They need to be free of oxygen so the anaerobes don’t die

What is the blood to broth ratio in blood culture bottles?

1:10

What are 2 specific media used for growth of AFB?

LJ and Middlebrook 7H11

What is the purpose of PEA agar?

Grows all GPC

Which streaking technique consist of 3 to 4 quadrants for isolation of bacteria?

Isolation Streak

Growth of normal stool bacteria on either a XLD or HE plate has what appearance?

Orange/Yellow Colonies

Specimens received on a swab which contains preservative should be stored how until inoculation onto media?

Room Temperature

What is the term used to describe no hemolysis of RBCs on a plate or broth?

Gamma

Other than the name of the patient, what is the most important information needed to correctly read-out a culture?

Culture or Specimen Source

Black colonies on HE is what organism?

Salmonella

Media with special ingredients to aid in growth of fastidious organisms is called:

Enriched Media

Colonies on a MAC plate which are lactose fermenters have what appearance?

Pink

1 drop of CSF for culture should be put onto what plate?

CHOC

Which media are capable of showing beta hemolysis?

SBA, CNA, and PEA

All collection containers MUST be sterile except for those containing what specimen type?

Stool

What is the purpose of SS agar?

Grows Salmonella/Shigella

Which media are capable of showing alpha hemolysis?

SBA, CNA, PEA, and CHOC

Anticoagulant in blood culture is what?

SPS

Why is a quantitative isolation streak used when inoculating urine specimens?

Allows the colonies to be counted

Media which aids in growth of 1 type of organism while inhibiting growth of another type is called:

Selective

If unable to immediately setup a CSF culture, how should it be stored?

Incubator

What is the purpose of TM agar?

Grows of Neisseria species

Inoculation of what culture type requires a calibrated loop to be used?

Urine

AFB media must be inoculated in the dark, due to production of what?

Formaldehyde

What type of culture would you use a selenite broth on?

Stool

What is the purpose of CNA agar?

Grows all GPC

An unpreserved urine should be stored how if it can’t be cultured immediately?

Refrigerated

Orange or yellow colonies on which media indicates normal stool bacteria?

XLD and HE

What is the purpose of CHOC agar?

Grows all bacteria including Haemophilus

What media is selective for growth of GPC?

CNA and PEA

What media is considered to be nonselective for growth of bacteria?

SBA

A sputum culture has growth of colonies into the 3rd or 4th quadrants. How should this be reported?

Moderate/Heavy Growth

What bacteria needs increased CO2 for growth called?

Capnophile

What temperature should be used to obtain growth for most bacteria associated with human infections?

35-37 degrees Celsius

A urine culture setup on SBA and MAC plates has growth on only the SBA plate after incubation. What does this mean?

UTI caused by a GPC