BIO 220 Module 12

serology

The branch of immunology that deals with in vitro diagnostic testing of serum.

Based on the principle that antibodies have extreme specificity for antigens, when exposed to its specific antibody, it will fit like a hand in a glove 

Phenotypic Characteristics if Identification

• Observation of the microbe's microscopic and

  macroscopic morphology, physiology, and

  biochemical properties

Immunologic Characteristics if Identification

Analysis of microbe using antibodies, or of patients'

antibodies using prepackaged antigens

Genotypic Characteristics if Identification

Analysis of microbe's DNA or RNA

ELISA (Enzyme-Linked Immunosorbent Assay) 

Sandwiching technique

conducted in microwells

using Ag, Ab, and a

secondary Ab to produce a

color change

Western Blot

Electrophoresis separates

proteins (either antigens or

antibodies) and then

labeled antibodies or

antigens are used for

detection.

Titer

concentration of antibodies in a sample; highest dilution of serum that still produces agglutination

Abbreviation for enzyme-linked immunosorbent assay, a very sensitive serological test used to detect antibodies in diseases such as AIDS.

ELISA