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How is cancer defined?
uncontrolled cell growth
ability to spread
How does cancer develop?
step wise process from multiple DNA mutations
mutations in oncogenes and/or tumor suppressor genes

What are oncogenes?
mutated proto-oncogene that promotes too much cell growth and division
protooncogenes regulate cell growth and division
like a GOF mutation; 1 copy enough to stimulate cancer


What are tumor suppressor genes
gene that controls/checks cell growth to ensure cell division occured properly
absense supports unregulated cell growth/divison
LOF; need two copies for cancer

Mutliple copies of a gene are created on a chromosome, causing cancer. Is it an oncogene or tumor-suppressor gene? Why?
oncogene
gene amplified - increases cell growth

How can translocation cause cancer-promoting mutations?
move chromosome pieces to a more active/inactive gene expression region
ex. proto-oncogene reciprocally translocated with another gene next to the enhancer. translocation causes GOF, overexpressing oncogene
ex. chromosome breaks in tumor suppressor enhancer, gene doesn’t get expressed
fusion gene - chromosome breaks and during repair joins part of one gene to part of another gene. The new hybrid gene may make an abnormal protein that promotes cancer
ex. overactive kinase activates everything

Are these likely causes of oncogenes or tumor suppressor genes causing cancer?
Large chromosomal deletion
Large chromosomal duplication
trisomy
monosomy
Large chromosomal deletion - tumor suppressor
Large chromosomal duplication - oncogene
trisomy - oncogene
monosomy - tumor suppressor
What would a point mutation GOF vs LOF cause in a proto-oncogene? What would a frameshift cause?
GOF - cancer
LOF - less cell growth
frameshift - likely LOF, less cell growth
What would a point mutation GOF vs LOF cause in a tumor suppressor gene?
GOF - more checked cell growth
LOF - cancer if only copy or copy in both alleles
frameshift; cancer if only copy or copy in both alleles