3A - B. Maturation and Morphology of Immature Granulocytes

Growth factors

The myeloid progenitor cell gives rise to a committed progenitor cell that is acted on by __________ to form granulocytes

Arrange the Immature granulocytes from earliest to oldest

Myeloblast, Promyelocyte, Myelocyte, Metamyelocyte, Band neautrophil

Myeloblast

Earliest recognizable granulocyte precursor

Metamyelocyte

Myeloblast

Band neutrophil

Promyelocyte

Myelocyte

10 – 18 µm

14 – 20 µm

10 – 15 µm

15 – 21 µm

12 – 18 µm

Metamyelocyte

Promyelocyte

Band neutrophil

Myelocyte

Myeloblast

N:C ratio 1.5:1

N:C ratio 3:1

N:C ratio 1:2

N:C ratio 2:1

N:C ratio 7:1 - 4:1

Band neutrophil

Promyelocyte

Metamyelocyte

Myeloblast

Myelocyte

Nucleus is “C” or “S” - shaped with coarse, clumped chromatin

Round/oval nucleus with slightly coarsening chromatin

Nucleus is indented in a kidney bean shape and has coarse, clumped chromatin

Round/oval nuclues with fine reddish-purple staining chromatin

Round nucleus with coarse chromatin

Myelocyte

Metamyelocyte, Band neutrophil

Myeloblast, Promyelocyte

Light blue to light pink cytoplasm

Cytoplasm is pink and filled with pale blue to pink specific/secondary granules (2 answers)

Dark blue cytoplasm (2 answers)

Promyelocyte

Myeloblast

Myelocyte

Cytoplasm has large, nonspecific/primary granules containing myeloperoxidase

No cytoplasmic granules

Cytoplasm has specific/secondary granules that contain hydrolytic enzymes, including alkaline phosphatase and lysozyme.

Myelocyte

Metamyelocyte

Band neutrophil

Nonspecific/primary granules are present and may still stain

Nonspecific/primary granules are present but usually do not stain

Nonspecific/primary granules are present but usually do not stain

Band neutrophil

Promyelocyte

Metamyelocyte

Myeloblast

Myelocyte

Makes up 12% of the nucleated cells in the bone marrow, and 0.5% of peripheral white blood cells (WBCs)

2–5% of the nucleated cells in the bone marrow

Makes up 16% of the nucleated cells in the bone marrow

1% of the nucleated cells in the bone marrow

Makes up 13% of the nucleated cells in the bone marrow

Myelocyte

Last stage capable of cell division

Band neutrophil

Nuclear indent is greater than half the width of a hypothetical round nucleus

Mature

Immature

Mature

Immature

Mature

Mature

Mature

Mature

Immature

Immature

Immature

Immature

Indicate wether it is an Immature or Mature Leukocyte

Nucleus round, lobulated, or segmented

High N:C ratio

Cytoplasm light blue (less RNA)

Chromatin fine and delicate

Cell becomes smaller

Chromatin coarse and clump

Nucleoli absent

Low N:C ratio

Nucleoli present

Cell is large

Nucleus round

Cytoplasm dark blue (rich in RNA)

Band neutrophil

Stored in the bone marrow and released when there is an increased demand.