Hematology 2 - Laboratory - Prussian Blue Reaction - Complete

Perl's reagent

Prussian blue reaction reagent

Potassium ferrocyanide-Hydrochloric acid

Perl's reagent chemical name

Hydrochloric acid

Perl's reagent component that causes the release of non-heme iron in hemosiderin?

Ferric iron

Other name for non-heme iron

Potassium ferrocyanide

Perl's reagent component that reacts with the liberated ferric iron to form a blue insoluble compound

Ferric ferrocyanide, Prussian blue

Name of blue insoluble compound formed after ferric iron reacts with potassium ferrocyanide

Neutral red

Prussian blue reaction counterstain

Methanol

Prussian blue reaction fixative

Bone marrow aspirate

Prussian blue reaction specimen of choice

To demonstrate non-heme iron or ferric iron, Evaluation of iron stores, Used as a gold standard of iron deficiency anemia

Prussian blue reaction usefulness (3)

Iron deficiency anemia

Prussian blue reaction is a gold standard for this condition

Low power objective

Objective used to scan bone marrow smear in Prussian blue reaction

100X

Magnification used to scan bone marrow smear in Prussian blue reaction

0, 1, 2, 3, 4

Numerical values used to report Prussian blue reaction

Absent or Depleted

Prussian blue reaction

Provide description of grading: 0

Decreased

Prussian blue reaction

Provide description of grading: 1

Adequate or Normal

Prussian blue reaction

Provide description of grading: 2

Moderately increased

Prussian blue reaction

Provide description of grading: 3

Markedly increased

Prussian blue reaction

Provide description of grading: 4

Iron deficiency anemia, Polycythemia vera, Myeloproliferative diseases, Pernicious anemia

Clinical significance for Prussian blue reaction if decreased results

Hemochromatosis, Megaloblastic anemia in relapse, Thalassemias, Chronic pancreatic insufficiency, Paroxysmal nocturnal hemoglobinuria

Clinical significance for Prussian blue reaction if increased results