1. BASIC PRINCIPLES AND PRACTICES

Panel

a set of related tests.

1. Actual value

2. Unit of expression

ENUMERATE

the two components of a quantitative laboratory result:

Système Internationale d’Unités (SI)

globally accepted and preferred system of units invthe laboratory

1. base

2. derived

3. non-SI

ENUMERATE

what are the subclassification of SI units

True

T or F

in reporting CC lab results mass of a substance is used rather than the SI units

closed reagent system

a type of reagent system wherein the analyzer only uses reagents approved or supplied by the analyzer manufacturer.

open reagent system

a type of reagent system that allows users to utilize reagents from different manufacturers

Analytic Reagent (AR) Grade

For trace metal analysis and preparation of standard solution

1. AR

2. ACS

3. “For laboratory use or ACS Standard-Grade Reference Materials”

what are the labels that should be printed in the storage containers if the chemical is analytic reagent?

Ultrapure Reagent

the chemical used in specific procedures such as chromatography, atomic absorption, immunoassays, and molecular diagnostics

1. HPLC (high performance liquid chromatography)

2. chromatographic

what are the labels that should be printed in the storage containers if the chemical is an ultrapure reagent?

1. research

2. analytical chemistry

if you’re using a Chemically Pure (CP)/ Pure Grade reagent, you must not use it in what fields in the laboratory since it is not recommended?

measurement of melting point or boiling point

how do you asses the purity of chemically pure/pure grade reagent?

Technical/ Commercial Grade

the reagent that is primarily used in manufacturing

False

T or F

you can use Technical/ Commercial Grade in the clinical laboratory

MATERIAL SAFETY DATA SHEET (MSDS)

A major source of safety information for employees

STANDARD SOLUTION/ STANDARDS

Solution containing known concentration of a particular chemical or analyte

STANDARD SOLUTION/ STANDARDS

Used to calibrate instruments used for colorimetric, electrochemical, turbidimetric, & other analytical measurements

• primary

• secondary

what are the two types of std sol’n?

PRIMARY STANDARD

Highly purified chemical that can be measured directly to produce substance of exact known concentration & purity

SECONDARY STANDARD

Substance of lower purity, with its concentration determined by comparison with primary standard

○ College of American Pathologists (CAP)

○ Clinical and Laboratory Standards Institute (CLSI)

ENUMERATE

two (2) organizations providing guidelines for proper chemical selection and reagent preparation

Water

Most frequently used reagent in clinical laboratory

○ Clinical laboratory reagent water (CLRW)

○ Special reagent water (SRW)

○ Instrument feed water

○ Water supplied by method manufacturer

○ Autoclave and wash water

○ Commercially bottled purified water

ENUMERATE

6 types of reagent grade water

Clinical laboratory reagent water (CLRW)

Can be of use in place of Type 1 and Type 2 water

Autoclave and wash water

Satisfactory replacement for Type 3 water

Type 1

For test methods or analytical procedures requiring minimum interference

Type 1

type of water used in Flame photometry, AAS (Atomic Absorption Spectrometry), blood gases and pH (analysis), enzyme studies, electrolyte testing, HPLC (high performance liquid chromatography), trace metal and iron studies

Type 2

type of water used in Hematology, Microbiology, Immunology, and Chemical analysis, Reagent, quality control, and standard preparation

Type 3

type of water used in UA (urinalysis), Parasitology, and Histology, Glassware washing

Prefiltration

uses activated charcoal to remove organic materials and a submicron filter or a filter that would remove substances larger than the filter’s pores

Reverse osmosis

High pressure water flow and a semi permeable membrane (acting as molecular filter)

Deionization

This method uses bead-like resin materials (ion exchange resins (cation resin and anion resin))