Chromatography

What is the mobile phase in chromatography?-The solvent moving through the column.

What is another name for the mobile phase?-Carrier.

Define mobile phase.-The solvent moving through the column.

What is the stationary phase in chromatography?-The substance that stays fixed inside the column.

What is another name for the stationary phase?-Adsorbent.

Define stationary phase.-The substance that stays fixed inside the column.

What is the eluent?-The fluid entering the column.

Define eluent.-The fluid entering the column.

What is the eluate?-The fluid exiting the column that is collected in flasks.

Define eluate.-The fluid exiting the column that is collected in flasks.

What is elution?-The process of washing out a compound through a column using a suitable solvent.

Define elution.-The process of washing out a compound through a column using a suitable solvent.

What is an analyte?-A mixture whose individual components must be separated and analyzed.

Define analyte.-A mixture whose individual components must be separated and analyzed.

Which chromatography component moves through the column?-Mobile phase.

Which chromatography component remains fixed inside the column?-Stationary phase.

What enters the chromatography column?-Eluent.

What exits the chromatography column?-Eluate.

What is the process of washing compounds out of a column called?-Elution.

What is the sample mixture being separated called?-Analyte.

Mobile phase is associated with what movement?-Moving through the column.

Stationary phase is associated with what movement?-Remaining fixed in the column.

What is the difference between eluent and eluate?-Eluent enters the column; eluate exits the column.

What is the difference between mobile phase and stationary phase?-The mobile phase moves through the column, while the stationary phase remains fixed.

A fluid is entering a chromatography column. What is it called?-Eluent.

A fluid is leaving a chromatography column. What is it called?-Eluate.

A chemist is separating the components of a mixture. What is the mixture called?-Analyte.

What chromatography term describes the washing of a compound through a column with solvent?-Elution.

How can you remember eluent versus eluate?-Eluent enters the column; eluate exits the column.

What is the stationary phase in paper chromatography?-Cellulose paper.

What is the mobile phase in paper chromatography?-Liquid solvent.

What is the basis of separation in paper chromatography?-Polarity of molecules.

How is the sample applied in paper chromatography?-The compound is spotted directly onto cellulose paper.

What is the stationary phase in thin layer chromatography?-Silica or alumina.

What is the mobile phase in thin layer chromatography?-Liquid solvent.

What is the basis of separation in thin layer chromatography?-Polarity of molecules.

How is the sample applied in thin layer chromatography?-The compound is spotted onto a thin layer of silica coated on glass.

What does TLC stand for?-Thin layer chromatography.

What is the stationary phase in liquid column chromatography?-Silica or alumina.

What is the mobile phase in liquid column chromatography?-Liquid solvent.

What is the basis of separation in liquid column chromatography?-Polarity of molecules.

How is the stationary phase arranged in liquid column chromatography?-A glass column is packed with a slurry of silica.

Which chromatography techniques separate molecules based on polarity?-Paper chromatography, thin layer chromatography, and liquid column chromatography.

What is the stationary phase in size exclusion chromatography?-Microporous silica beads.

What is the mobile phase in size exclusion chromatography?-Liquid solvent.

What is the basis of separation in size exclusion chromatography?-Molecular size.

Which molecules become trapped in the pores during size exclusion chromatography?-Small molecules.

Which molecules elute first in size exclusion chromatography?-Large molecules.

Why do large molecules elute first in size exclusion chromatography?-They cannot enter the pores and flow around the beads.

What is unique about size exclusion chromatography?-There is no physical or chemical interaction between the analyte and stationary phase.