Studied by 67 peopleClothing regulations
Suitable clothing must be worn.
NOT ALLOWED: Open-toed shoes, short shorts, short shirts, skirts, and tank tops
Use of Lab other than Class times
Lab can be used other than class times for class lab work during lab open hours with notification made to lab supervisors or professor
work needs to be done in other than scheduled areas/ no disruptive activity
Priority of lab use is given to those students who are scheduled to use the lab at that time.
Safety/Lab Policy exam
each semester take safety test and pass > 85% 2 chances for advanced students 3 chances for EM 70 students
REFERENCES Bozolla Chapter 20
This Lab policy document
MSDS Sheet
Student should be aware of and utilize all necessary safety precautions when using chemicals and equipment in the lab
Student is responsible for reading the MSDS sheet associated with chemical found in the laboratory
DO not remove any MSDS sheets from the notebook
read MSDS before asking questions from LAB SUPES/Instructor
Safe Work Habits
Students are expected to ACT and OPERATE in a SAFE MANNER at all times in the LAB
Identification of Safety Provisions
Identify all exits eye washes safety shower fire extinguishers MSDS Manual
What are the regulations on safety glasses?
Wearing safety glasses mandatory
While looking though the binoculars of the light microscopes glasses do NOT have to be worn. (Must be worn at all other times)
Safety glasses must be worn when using?
hood areas for chemicals
Violation of Safety Glasses
Will be asked to leave and not return for 1 hour
Disposable Gloves usage when?
using RESIN and other potentially toxic chemicals or materials
What care must be taken with soiled gloves
Care not to contaminate objects Care not to contaminate yourself
T or F
Any Biological agent was handled during use (gloves), they must be disposed of in the BIOHAZARD container
TRUE
LAB exits are for what usage
EMERGENCY EXIT, ONLY FOR EMERGENCIES
Dispose of Broken Glass (EXAMPLES)
Broken Glassware Glass Knives used syringes needles in their separate appropriate RED "sharps" containers
Where is RED sharp containers located?
located either by sink or by ultramicrotomes for GLASS KNIVES
LAB ACCIDENT
in case of a chemical spill, lab accidents, or fire
NOTIFY lab supervisor or instructor IMMEDIATELY before taking any further action
If you must leave, you are to WARN other students in the AREA of the DANGER before leaving the area
Container Labeling
ALL glassware/containers containing liquids/solids must be labeled while in, use even beakers of water
Use white tape or a Sharpie to label container with name of chemical, date, and student's name and class
INITIALS ARE NOT ACCEPTABLE
T OR F
Beakers are not to be used to STORE any solutions
TRUE
T or F SMOKING IS ALLOWED IN THE CAMPUS
FALSE
NO, NOT ALLOWED ANYWHERE IN THE CAMPUS
T or F
Unauthorized personnel is not allowed in the lab without supervision
TRUE
Every student pass EM 70 must purchase one copy each of?
SOPs for Electron Microscopes Biological and Materials Lab Preparation
T or F
After initial purchase is made, updated packages must be purchase (SOPs and Lab Preparation)
TRUE
T or F
Students must have in their possession their copy of the equipment SOPs in order to be ABLE to use the equipment
TRUE
T or F
Specialty items Glass knives, Pella Ruler, fine tipped forceps, grids etc. Must be purchased at bookstore
TRUE
T or F
Personal items may be kept in the lab
FALSE
Box must leave when you do
T or F
Students must clearly label all personal materials
TRUE
T or F
Lab glassware and materials should be kept in student's box
FALSE
Cannot be kept in your student box
T or F
NO chemicals should be kept in student's box
TRUE
T or F
Instructors and Lab SUPES have the RIGHT to inspect student's boxes at any time
TRUE
T or F
Lab/microscopy lab is RESPONSIBLE for personal materials/items
FALSE
YOU are responsible for your personal items/materials
T or F
Students are required to remove their personal belongings by the final day of classes each semester
TRUE
T or F
Food and Drinks are allowed in CMAS
FALSE
Not allowed inside CMAS
T or F
Can dispose empty food and drink containers inside the building's trashcans
F
Dispose OUTSIDE of the building
T or F
Backpacks are NOT allowed in any labs and microscope rooms
TRUE
T or F
No books, lab equipment, or materials including chemicals are to be removed from the microscopy lab that is not personally owned
TRUE
T or F
Books are for use in the lab ONLY
TRUE
Who is responsible for glassware cleaning?
Students are required to properly clean and put away all glassware and other materials used in their labs
T or F
do NOT leave dishes hanging on racks if DRY
TRUE
T or F
Before washing. Do not remove all tapes or labels
FALSE
Remove all tapes or labels before washing
T or F
Methanol or Propanol can be used to WIPE off labels put on with a sharpie
TRUE
T or F
ENTIRE class is responsible for the cleanliness of the lab after the leave
TRUE
Labs found left in an UNACCEPTABLE manner will be closed to student use the following day
T or F
Each lab class will have 2 Students assigned per lab session that are RESPONSIBLE for clean up of that session
TRUE
Instructor will post the names of those students responsible and will be checked for cleanliness before the students may leave
T or F
Students should NOT attempt to operate any equipment in the lab WITHOUT prior training or AUTHORIZATION by instructor/LAB SUPE
TRUE
T or F
Students must properly LOG their use of equipment indicating the requested information.
TRUE
Electron Microscopes Vacuum evaporator Sputter coaters Ultramicrotomes etc...
T or F
Violation of 42 and 43 will result in loss of privilege of using the equipment.
TRUE
T or F
Although microscope sign ups are posted on the bulletin board, students can sign themselves up for microscope times
FALSE
Students must contact Lab SUPES , who will sign them up
T or F
Microscope will be released to another if student is 10 minutes LATE changes to 5 minutes for the LAST 6 WEEKS of the semester
TRUE
T or F
Start SHUTDOWN procedure 10 min prior to the end of the SESSION
so you have the SAMPLE out of the microscope and out of the SPECIMEN holder
TRUE
T or F
The student at fault will forfeit their TBA time if found any student is using/running over another student's TBA TIME
TRUE
T or F
You can trade TBA times if you miss your TBA with another student's schedule for that week
TRUE
notify lab supe of exchange
T or F
It's important you do not miss the first 7 weeks of TBA TIME
TRUE
Student will be dropped if the miss 3 individual TBAs (unexcused)
T or F
Students who are more than 10 minutes late for TBA will be excused
FALSE
You will be considered absent (unexcused)
T or F
Students must only have a copy of SOPs to use the equipment
FALSE
need Lab Book and current copy of SOPs to use the equipment you will not be allowed to use the equipment if you don't have both of those items
T or F
If you need help you should ask help from an advanced student
FALSE
ask an instructor or lab supe for help not students
How many students are allowed in a room for TBA time?
Only student should be you
Who has equipment priority?
Student who has class at that time or Student outside of class schedule time
Student who has class at that time has higher priority.
Students outside of class schedule can use the equipment, but they must not disturb the class
T or F
Students must pass an oral alignment test for use of both TEM and SEM if directed by Professor
FALSE
Students must pass a written alignment test for use of both TEM and SEM if directed by Professor
T or F
Students must have current SOPs for all microscopes.
TRUE
Students must be _______ each semester during their scheduled class time for independent use
Students must be [checked out] each semester during their scheduled class time for independent use
T or F
Using the microscope is not a privilege
FALSE
Microscope usage is a privilege
What are the consequences/types of improper microscope usage?
If you miss too many microscope training times If you are found using the microscope for techniques for which you have not been checked out If you repeatedly abuse the microscope by using it improperly If you are found on any microscope without permission
Instructor/Lab supes have the authority to suspend your privileges
Microscope time is suspended and will have to suffer the consequences of not having that microscope work done
T or F
ALL images captured on any TEM/SEM must be stored on the server
TRUE
T or F
Students are permitted to plug any external storage devices into the computers running the imaging system
FALSE
Can only be downloaded in computers found in the library (CMAS 105)
T or F
Image files must be placed on the server using the correct file name format
TRUE
Incorrect name file will be deleted
T or F
All digital images must be logged into the digital image logbook found in each microscope room
TRUE
Students will NOT receive credit for assignments without this documentation.
Refrigerators and freezers in 131 are for _____ use
Chemical
T or F
All materials must be capped and then additionally covered with Parafilm
True
T or F
All vials containing osmium must be protected once
FALSE
ALL vials containing OSMIUM must be DOUBLE PROTECTED
How must all materials in the refrigerator be labeled?
with white labeling tape indicating:
class name, student's full name, specific name of solution (ex. 3% glutaraldehyde in 0.1 Cacodylate; stating glutaraldehyde is not sufficient) , and date
REMEMBER: if this info is not on the container it will be removed from the refrigerator
T or F
Materials should be in the refrigerator only as long as it is useful for COURSE and must be properly DISPOSED of before end of semester
TRUE
GRADES will not be turned in for students whose materials remain in the refrigerator without authorization after the end of the semester as this is considered a class requirement.
Storage containers types: must not store materials in?
Volumetric flask graduated cylinders Expensive measuring containers
T or F
Magnetic Stirrers must be removed from flasks or beakers before storing
TRUE
Magnetic Stirrers are to be ____________
cleaned, dried, and returned to their proper location IMMEDIATELY after use
T or F
Microwave should be used for anything to be consumed
TRUE ( NOT SURE WORDING SOUNDS WEIRD)
MAYBE ALTERNATIVE: Microwave should not be used for anything to be consumed
Pella Industrial Microwaves should be used for _____
preparation protocols
T or F
Do NOT change/set new, computer programmed times already existing on Pella Microwaves unless authorized by Professor
TRUE
T or F
ALL hazardous chemicals MUST be used in the fume hood
TRUE
No chemicals are to be _____ of down the hood ____
No chemicals are to be [disposed] of down the hood [drain]
T or F
Fume hoods are to remain off after usage
FALSE
Fume hoods are to remain ON, at ALL times
When fume hood is in use...
the sash or front pull down "door" should be mostly closed except for space to allow room for your hands
T or F
There are 12 fume hoods in CMAS
TRUE
Where are the Fume Hoods located?
4 in 131: UTILITIES LAB
4 in 130: GENERAL MICROSCOPY LAB
1 in 128: ADVANCED BIOLOGICAL PREPARATION LAB
2 in 126: MATERIALS PREPARATION ROOM
1 IN 125: MAINTENACE ROOM
T or F
11 FUME HOODS ARE ACCESSIBLE TO STUDENTS
TRUE
T or F
All waste chemicals must be disposed of in appropriate waste bottles in the hood
TRUE
If you cannot find a waste bottle or if it is full, do NOT make a new waste bottle. Special bottles and labels must be used
T or F
Students are NOT to begin any protocol without a waste bottle and NOT make their own waste bottle
TRUE
Only LAB SUPE is trained for waste containers
Can you mix chemical waste?
NO, do NOT mix chemical waste unless indicated on waste bottle
Why minimize waste solutions?
Saves chemicals and minimizes waste disposal, which is expensive
Should waste containers be left under any hood or on the counter?
Waste containers are NOT to be left under any hood or counter for any reason after 4:30 pm
Where should acid waste containers be placed?
In the cabinet under the hood in room 131
Where should all other waste containers be placed?
in the cabinet under the hood in room 130
All computers are for?
Student related work and checking student's email ONLY
Can you use the computers in room 102?
These computers remain off unless instructor requires
T or F
before using a diamond knife, student must be checked out on a Training Diamond Knife
TRUE
After use student must fill in logbook with requested information, and properly clean the diamond knife as demonstrated during training
T or F
When ready to turn diamond knife in, student should have CLEANING completed, diamond under stereomicroscope with a beaker of water and cleaning stick ready
TRUE
Lab Supes/Instructor/Designated Person MUST examine knife before returning
Diamond Knives must be TURNED IN by the USER personally to Lab Supes/Instructor/Designated Person
T or F
One person can give the diamond knife to another person
FALSE
You cannot give the diamond to another person. It must be given back and inspected by Lab Supe or Instructor
100% Ethanol must ______
be requested from supervisor
Do NOT make up dehydration solutions from ____ ethanol, but rather use ___, except, of course, for the final dehydration in ____
Do NOT make up dehydration solutions from [100%] ethanol, but rather use [95%], except, of course, for the final dehydration in [100%]
Students who need to use _____ or ______ need to first see lab supes before use. Do NOT removed materials from the chemical cabinet
Students who need to use [stock chemicals] or [solvents] need to first see lab supes before use. Do NOT removed materials from the chemical cabinet
T or F
Do NOT open a new bottle of chemical or solvent before getting lab supe authorization
TRUE
CMAS Phones REGULATIONS
not for student use unless authorized Do NOT answer phone unless authorized Message machine and phone will ring over to it after 2 or 4 rings do NOT give out the lab number to receive phone calls except for EMERGENCIES
Note
Flashcard