MICROSCOPY LAB USE POLICIES

Clothing regulations

Suitable clothing must be worn.

NOT ALLOWED: Open-toed shoes, short shorts, short shirts, skirts, and tank tops

Use of Lab other than Class times

Lab can be used other than class times for class lab work during lab open hours with notification made to lab supervisors or professor

work needs to be done in other than scheduled areas/ no disruptive activity

Priority of lab use is given to those students who are scheduled to use the lab at that time.

Safety/Lab Policy exam

each semester take safety test and pass > 85%
2 chances for advanced students
3 chances for EM 70 students

REFERENCES
Bozolla Chapter 20

This Lab policy document

MSDS Sheet

Student should be aware of and utilize all necessary safety precautions when using chemicals and equipment in the lab

Student is responsible for reading the MSDS sheet associated with chemical found in the laboratory

DO not remove any MSDS sheets from the notebook

read MSDS before asking questions from LAB SUPES/Instructor

Safe Work Habits

Students are expected to ACT and OPERATE in a SAFE MANNER at all times in the LAB

Identification of Safety Provisions

Identify all exits
eye washes
safety shower
fire extinguishers
MSDS Manual

What are the regulations on safety glasses?

Wearing safety glasses mandatory

While looking though the binoculars of the light microscopes glasses do NOT have to be worn. (Must be worn at all other times)

Safety glasses must be worn when using?

hood areas for chemicals

Violation of Safety Glasses

Will be asked to leave and not return for 1 hour

Disposable Gloves usage when?

using RESIN and other potentially toxic chemicals or materials

What care must be taken with soiled gloves

Care not to contaminate objects
Care not to contaminate yourself

T or F

Any Biological agent was handled during use (gloves), they must be disposed of in the BIOHAZARD container

TRUE

LAB exits are for what usage

EMERGENCY EXIT, ONLY FOR EMERGENCIES

Dispose of Broken Glass (EXAMPLES)

Broken Glassware
Glass Knives
used syringes
needles
in their separate appropriate RED "sharps" containers

Where is RED sharp containers located?

located either by sink or by ultramicrotomes for GLASS KNIVES

LAB ACCIDENT

in case of a chemical spill, lab accidents, or fire

NOTIFY lab supervisor or instructor IMMEDIATELY before taking any further action

If you must leave, you are to WARN other students in the AREA of the DANGER before leaving the area

Container Labeling

ALL glassware/containers containing liquids/solids must be labeled while in, use even beakers of water

Use white tape or a Sharpie to label container with
name of chemical, date, and student's name and class

INITIALS ARE NOT ACCEPTABLE

T OR F

Beakers are not to be used to STORE any solutions

TRUE

T or F
SMOKING IS ALLOWED IN THE CAMPUS

FALSE

NO, NOT ALLOWED ANYWHERE IN THE CAMPUS

T or F

Unauthorized personnel is not allowed in the lab without supervision

TRUE

Every student pass EM 70 must purchase one copy each of?

SOPs for Electron Microscopes
Biological and Materials Lab Preparation

T or F

After initial purchase is made, updated packages must be purchase (SOPs and Lab Preparation)

TRUE

T or F

Students must have in their possession their copy of the equipment SOPs in order to be ABLE to use the equipment

TRUE

T or F

Specialty items
Glass knives, Pella Ruler, fine tipped forceps, grids etc. Must be purchased at bookstore

TRUE

T or F

Personal items may be kept in the lab

FALSE

Box must leave when you do

T or F

Students must clearly label all personal materials

TRUE

T or F

Lab glassware and materials should be kept in student's box

FALSE

Cannot be kept in your student box

T or F

NO chemicals should be kept in student's box

TRUE

T or F

Instructors and Lab SUPES have the RIGHT to inspect student's boxes at any time

TRUE

T or F

Lab/microscopy lab is RESPONSIBLE for personal materials/items

FALSE

YOU are responsible for your personal items/materials

T or F

Students are required to remove their personal belongings by the final day of classes each semester

TRUE

T or F

Food and Drinks are allowed in CMAS

FALSE

Not allowed inside CMAS

T or F

Can dispose empty food and drink containers inside the building's trashcans

F

Dispose OUTSIDE of the building

T or F

Backpacks are NOT allowed in any labs and microscope rooms

TRUE

T or F

No books, lab equipment, or materials including chemicals are to be removed from the microscopy lab that is not personally owned

TRUE

T or F

Books are for use in the lab ONLY

TRUE

Who is responsible for glassware cleaning?

Students are required to properly clean and put away all glassware and other materials used in their labs

T or F

do NOT leave dishes hanging on racks if DRY

TRUE

T or F

Before washing. Do not remove all tapes or labels

FALSE

Remove all tapes or labels before washing

T or F

Methanol or Propanol can be used to WIPE off labels put on with a sharpie

TRUE

T or F

ENTIRE class is responsible for the cleanliness of the lab after the leave

TRUE

Labs found left in an UNACCEPTABLE manner will be closed to student use the following day

T or F

Each lab class will have 2 Students assigned per lab session that are RESPONSIBLE for clean up of that session

TRUE

Instructor will post the names of those students responsible and will be checked for cleanliness before the students may leave

T or F

Students should NOT attempt to operate any equipment in the lab WITHOUT prior training or AUTHORIZATION by instructor/LAB SUPE

TRUE

T or F

Students must properly LOG their use of equipment indicating the requested information.

TRUE

Electron Microscopes
Vacuum evaporator
Sputter coaters
Ultramicrotomes
etc...

T or F

Violation of 42 and 43 will result in loss of privilege of using the equipment.

TRUE

T or F

Although microscope sign ups are posted on the bulletin board, students can sign themselves up for microscope times

FALSE

Students must contact Lab SUPES , who will sign them up

T or F

Microscope will be released to another if
student is 10 minutes LATE
changes to 5 minutes for the LAST 6 WEEKS of the semester

TRUE

T or F

Start SHUTDOWN procedure 10 min prior to the end of the SESSION

so you have the SAMPLE out of the microscope and out of the SPECIMEN holder

TRUE

T or F

The student at fault will forfeit their TBA time if found
any student is using/running over another student's TBA TIME

TRUE

T or F

You can trade TBA times if you miss your TBA with another student's schedule for that week

TRUE

notify lab supe of exchange

T or F

It's important you do not miss the first 7 weeks of TBA TIME

TRUE

Student will be dropped if the miss 3 individual TBAs (unexcused)

T or F

Students who are more than 10 minutes late for TBA will be excused

FALSE

You will be considered absent (unexcused)

T or F

Students must only have a copy of SOPs to use the equipment

FALSE

need Lab Book and current copy of SOPs to use the equipment
you will not be allowed to use the equipment if you don't have both of those items

T or F

If you need help you should ask help from an advanced student

FALSE

ask an instructor or lab supe for help not students

How many students are allowed in a room for TBA time?

Only student should be you

Who has equipment priority?

Student who has class at that time
or
Student outside of class schedule time

Student who has class at that time has higher priority.

Students outside of class schedule can use the equipment, but they must not disturb the class

T or F

Students must pass an oral alignment test for use of both TEM and SEM if directed by Professor

FALSE

Students must pass a written alignment test for use of both TEM and SEM if directed by Professor

T or F

Students must have current SOPs for all microscopes.

TRUE

Students must be _______ each semester during their scheduled class time for independent use

Students must be [checked out] each semester during their scheduled class time for independent use

T or F

Using the microscope is not a privilege

FALSE

Microscope usage is a privilege

What are the consequences/types of improper microscope usage?

If you miss too many microscope training times
If you are found using the microscope for techniques for which you have not been checked out
If you repeatedly abuse the microscope by using it improperly
If you are found on any microscope without permission

Instructor/Lab supes have the authority to suspend your privileges

Microscope time is suspended and will have to suffer the consequences of not having that microscope work done

T or F

ALL images captured on any TEM/SEM must be stored on the server

TRUE

T or F

Students are permitted to plug any external storage devices into the computers running the imaging system

FALSE

Can only be downloaded in computers found in the library (CMAS 105)

T or F

Image files must be placed on the server using the correct file name format

TRUE

Incorrect name file will be deleted

T or F

All digital images must be logged into the digital image logbook found in each microscope room

TRUE

Students will NOT receive credit for assignments without this documentation.

Refrigerators and freezers in 131 are for _____ use

Chemical

T or F

All materials must be capped and then additionally covered with Parafilm

True

T or F

All vials containing osmium must be protected once

FALSE

ALL vials containing OSMIUM must be DOUBLE PROTECTED

How must all materials in the refrigerator be labeled?

with white labeling tape indicating:

class name, student's full name, specific name of solution (ex. 3% glutaraldehyde in 0.1 Cacodylate; stating glutaraldehyde is not sufficient) , and date

REMEMBER: if this info is not on the container it will be removed from the refrigerator

T or F

Materials should be in the refrigerator only as long as it is useful for COURSE and must be properly DISPOSED of before end of semester

TRUE

GRADES will not be turned in for students whose materials remain in the refrigerator without authorization after the end of the semester as this is considered a class requirement.

Storage containers types:
must not store materials in?

Volumetric flask
graduated cylinders
Expensive measuring containers

T or F

Magnetic Stirrers must be removed from flasks or beakers before storing

TRUE

Magnetic Stirrers are to be ____________

cleaned, dried, and returned to their proper location IMMEDIATELY after use

T or F

Microwave should be used for anything to be consumed

TRUE ( NOT SURE WORDING SOUNDS WEIRD)

MAYBE ALTERNATIVE: Microwave should not be used for anything to be consumed

Pella Industrial Microwaves should be used for _____

preparation protocols

T or F

Do NOT change/set new, computer programmed times already existing on Pella Microwaves unless authorized by Professor

TRUE

T or F

ALL hazardous chemicals MUST be used in the fume hood

TRUE

No chemicals are to be _____ of down the hood ____

No chemicals are to be [disposed] of down the hood [drain]

T or F

Fume hoods are to remain off after usage

FALSE

Fume hoods are to remain ON, at ALL times

When fume hood is in use...

the sash or front pull down "door" should be mostly closed except for space to allow room for your hands

T or F

There are 12 fume hoods in CMAS

TRUE

Where are the Fume Hoods located?

4 in 131: UTILITIES LAB

4 in 130: GENERAL MICROSCOPY LAB

1 in 128: ADVANCED BIOLOGICAL PREPARATION LAB

2 in 126: MATERIALS PREPARATION ROOM

1 IN 125: MAINTENACE ROOM

T or F

11 FUME HOODS ARE ACCESSIBLE TO STUDENTS

TRUE

T or F

All waste chemicals must be disposed of in appropriate waste bottles in the hood

TRUE

If you cannot find a waste bottle or if it is full, do NOT make a new waste bottle. Special bottles and labels must be used

T or F

Students are NOT to begin any protocol without a waste bottle and NOT make their own waste bottle

TRUE

Only LAB SUPE is trained for waste containers

Can you mix chemical waste?

NO, do NOT mix chemical waste unless indicated on waste bottle

Why minimize waste solutions?

Saves chemicals and minimizes waste disposal, which is expensive

Should waste containers be left under any hood or on the counter?

Waste containers are NOT to be left under any hood or counter for any reason after 4:30 pm

Where should acid waste containers be placed?

In the cabinet under the hood in room 131

Where should all other waste containers be placed?

in the cabinet under the hood in room 130

All computers are for?

Student related work and checking student's email ONLY

Can you use the computers in room 102?

These computers remain off unless instructor requires

T or F

before using a diamond knife, student must be checked out on a Training Diamond Knife

TRUE

After use student must fill in logbook with requested information, and properly clean the diamond knife as demonstrated during training

T or F

When ready to turn diamond knife in, student should have CLEANING completed, diamond under stereomicroscope with a beaker of water and cleaning stick ready

TRUE

Lab Supes/Instructor/Designated Person MUST examine knife before returning

Diamond Knives must be TURNED IN by the USER personally to Lab Supes/Instructor/Designated Person

T or F

One person can give the diamond knife to another person

FALSE

You cannot give the diamond to another person. It must be given back and inspected by Lab Supe or Instructor

100% Ethanol must ______

be requested from supervisor

Do NOT make up dehydration solutions from ____ ethanol, but rather use ___, except, of course, for the final dehydration in ____

Do NOT make up dehydration solutions from [100%] ethanol, but rather use [95%], except, of course, for the final dehydration in [100%]

Students who need to use _____ or ______ need to first see lab supes before use. Do NOT removed materials from the chemical cabinet

Students who need to use [stock chemicals] or [solvents] need to first see lab supes before use. Do NOT removed materials from the chemical cabinet

T or F

Do NOT open a new bottle of chemical or solvent before getting lab supe authorization

TRUE

CMAS Phones REGULATIONS

not for student use unless authorized
Do NOT answer phone unless authorized
Message machine and phone will ring over to it after 2 or 4 rings
do NOT give out the lab number to receive phone calls except for EMERGENCIES