Unit 1- lab Endospore Stain Lab
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30 Terms
Who developed the original Acid-Fast Stain in 1882?
Paul Ehrlich
What type of stain is the Acid-Fast Stain?
differential stain
What unique substance is in the cell wall of acid-fast bacteria?
Mycolic acid
What properties does this substance give these bacteria?
List two acid fast genera and the diseases associated with them:
Mycobacterium and Nocardia
What is the primary stain in the Ziel-Neelsen acid-fast stain method?
Carbolfuchsin
How do we drive this stain into the acid-fast cell wall?
heat the cells with steam
What is the decolorizer in the acid-fast stain?
acid alcohol
What is the counter stain in the acid-fast stain?
methylene
What color will acid-fast cells appear? What about non-acid-fast cells?
white
What do you call an actively growing and metabolizing bacterial cell?
vegetative cell
What do you call a dormant resistant structure that some bacteria make in times of stress?
endospores
List the things that this structure is resistant to:
heat desiccation, radiation, disinfectants
What do you call the state of no measurable metabolic activity that an endospore exists in
cryptobiotic
How do we drive stain into an endospores tough coat?
steam
What do you call it when a spore is in the middle of a cell?
central spore
What do you call it when a spore is located at the end of the cell?
terminal spore
What do you call it when a spore is not quite in the middle, and not quite at the end?
subterminal spore
What do you call it when the cell swells up around the spore?
terminal with enlarged sporangium cell
Which spore-forming species causes tetanus, a deadly pathogen that causes muscles to lock or spasm?
botulism
Which spore-forming species causes gas gangrene and a certain type of food poisoning?
clostridium tetani
Which spore-forming species causes gas gangrene and a certain type of food poisoning?
Clostridium
Which spore-forming species causes anthrax?
Bacillus anthracis
Which spore-forming species produces the antibiotic bacitracin?
bacillus cereus
What dye is steamed into the cells to stain the endospores?
malachite green
What is used to decolorize?
water
What color will the vegetative cells be?
safranin