journal club 4 + mmg2040

Differentiate v-SRC

v-SRC: viral sarc

• picked up chicken gene and carries it around itself

• protein kinase activity is always on —> phosphorylating many things

  • means cells are unregulated, go into uncontrolled cycle —> causes cancer

Define: sarcoma

a form of cancer that develops in the connective tissues of the body, including bones, muscles, fat, nerves, tendons, and blood vessels

Describe thin layer electrophoresis (paper electrophoresis)

paper in between two buffer wells —> Separation depends on the net charge of the molecule, which influences its speed of migration towards the opposite electrode (cathode or anode)

• separates by size, charge, etc.

• The medium is saturated with buffer and connected to reservoirs.

• The sample is spotted in the center or end of the strip.

• A voltage is applied, causing migration for a specific duration.

• Separated spots are visualized with staining or specific detection reagents.

c-SRC genes

c-SRC: cellular sarc

• og gene that virus picked up from

• human and chicken sarc has extra c-terminus stuff to keep kinase strictly controlled —> y5230 + y527