Lab exam 205-2

What is Bacillus anthracis classified as in terms of biosafety?

It is a Hazard group 3 pathogen.

How does Bacillus anthracis spread?

It spreads via spores, typically through grazing animals or air.

What is the purpose of a Biosafety Cabinet?

It provides protection for operators, work, and both by creating a controlled environment.

What does BSL-3 lab PPE protocols include?

It includes proper donning and doffing procedures for personal protective equipment.

What is the function of negative pressure flow in a laboratory?

It prevents airborne agents from escaping.

What are the key components of the 'buddy system' in lab safety?

It involves having partners for safety checks, sign-in protocols, and training.

What are the general steps for ensuring sterility of samples before removal from the lab?

It includes fumigation and lab decontamination procedures.

What are the main techniques used for sterilizing medium and containers?

Autoclaving procedures.

What is the significance of the Bradford Assay?

It is a colorimetric method for determining protein content.

What distinguishes Gram-positive from Gram-negative bacteria?

They differ in their cell wall structure and thickness.

What are the phases of bacterial growth?

Lag, Log, Stationary, and Death phases.

What does LTLT stand for in pasteurization?

Low-Temperature Long-Time.

How does UHT sterilization work?

It involves ultra-high temperature treatment for sterilization.

What factors affect microbial growth?

Temperature, pH, nutrients, and other environmental conditions.

What is the purpose of Salmonella Shigella Agar (SSA)?

It is used for isolating Salmonella species.

What is the difference between food intoxication and food infection?

Food intoxication is caused by toxins produced by microbes, while food infection involves the ingestion of live bacteria.

What is a microliter pipette used for?

It is used for precise measurement of small volumes of liquid.

What is the role of flagella in bacteria?

It assists in motility and movement towards nutrients.

What does the term 'heat treatment' refer to in food preservation?

It refers to processes like pasteurization and sterilization to eliminate pathogens.