Phlebotomy Part 1

Arteries

Carry oxygenated blood away from the heart, deeper, pulsation

Veins

Carry deoxygenated blood to the heart, superficial (surface)

Capillaries

Carry both deoxygenated and oxygenated blood and connect to arteries and veins (Skin)

Median

Middle of the AC (antecubital fossa) (1st choice)

Cephalic

Lateral aspect (away, 2nd choice)

Basilic

Inner aspect (last choice, over brachial artery, nerves)

Whole Blood is composed of….

Formed elements and plasma

Red Blood Cells (RBC)

Erythrocyte, Carries oxygen, hemoglobin= expands oxygen

White Blood Cells (WBC)

Leukocyte, fights infection, most numerous WBC is neutrophil

Platelets (PLT)

Thrombocyte, Clotting

Once blood is removed, the blood coagulates and changes composition of the blood to…

Formed elements and serum (removed) (clots)

Anticoagulant

Prevents the blood from clotting

1. Blood culture/Yellow

SPS

(Order of draw)

2. Light blue

Sodium Citrate

3. Red (SERUM)

None

4. Tiger/ Gold (SST) (SERUM)

None, Gel barrier

5. Green

Heparin

6. Pink/Purple

EDTA

7. Gray

Sodium Fluoride and Potassium Oxalate

8. Yellow

ACD

Red and Tiger/Gold

does NOT have anticoagulate, takes 30 minutes to clot, when centrifuge (spin for 10 min) serum will be at the top, gel barrier and then clot at the bottom

All other tubes (Blood culture/ yellow, light blue, green, pink/purple, gray and yellow)

Has anticoagulate, leave alone= whole blood, spin (centrifuge) = Plasma

Tube with an anticoagulant (prevents clotting) and centrifuge (spin) will yield?

Plasma

Tube with an anticoagulant (prevent clotting) and not centrifuge (no spin) will stay as?

Whole blood

Tube without an anticoagulant , clots, and then centrifuge will yield?

serum (30 mins clot wait time)