Oxidation/Fermentation and Multiple Test

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Last updated 4:12 AM on 4/15/26
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5 Terms

1
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Oxidative Fermentation

Yellow (with no mineral oil)→ Green (with mineral oil) = Strict Aerobe

Yellow (with no mineral oil)→ Yellow (with mineral oil= Facultative Anaerobe

Green (with no mineral oil)→ Green (with mineral oil)= Can’t be determined

2
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Litmus Milk Broth

Substrates: Lactose, Casein

Enzymes: Lactase, Caseinase

Minimum Incubation: 48hrs+

Reagents: Litmus indicator

3
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Litmus Milk Broth test results:

Positive:

  • Acid (pink)

  • Alkaline (light to dark blue)

  • Litmus reduction (white)

  • Curd (milk changes to solid state)

  • Proteolysis (decrease in milk turbidity more water appearance)

Negative: No color change (purple)

4
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SIMs (sulfide, indole, motility)

Substrates: Cystein, tryptophan, None

Enzymes: Cystein desulfurase, tryptophanase, none

Minimum incubation: 48 hrs

Reagents: Iron, Kovac’s, none

5
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SIMs Test results:

Positive:

  • H2S (blackens medium)

  • Indole(red ring)

  • Motility (turbid throughout

Negative:

  • H2S- (no blackening)

  • Indole (no red ring)

  • No motility (growth only in inoculation line)