microscope quiz

Eyepiece/Ocular Lens

What you look through, magnifies 10x

Revolving Nosepiece

Switches between objective lenses

Objective Lenses

Magnifies specimen (low, 4x) (medium, 10x) (high, 40x)

Stage

Where you put the specimen

Stage clips

Holds the specimen in place

Lamp

Supples light to view the specimen

Diaphragm

Opens/closes to control how much light passes through the specimen

Coarse Adjustment Knob

Moves stage up/down to bring specimen roughly into focus

Fine adjustment knob

Brings specimen into sharper focus

Arm

What you hold when carrying a microscope

Base

Bottom of the microscope where you hold when carrying.

Eyepiece

Arm

Coarse Adjustment Knob

Fine Adjustment Knob

Revolving Nosepiece

Objective Lenses

Stage

Stage Clips

Diaphragm

Base

Lamp

Aristole (384 - 322 BC)

The Father of Biology; one of the first to use the scientific method.

Janssn Bothers (1590)

Dutch eyeglass makers. They made the first microsscope.

Galileo (1609)

Improved the microscope with better magnification

Schleidan and Schwann (1830)

Observed cells and came up with the idea that all living things are composed of cells.

Van Leeuwenhoek (1674)

First person to observe the movement of living cells

Hooke (1665)

Added lensess and light to Janssens design and came up with the idea of cells.

Virchow (1885)

Came up with the idea that new cellss arise fom pre-existing cells

Ernst and Knoll (1931)

Invented electron microscope

Zernik (1953)

Won nobel prize for phase contast method

The Cell Theory

1. All living thing are made up of cells

2. The cell is the smallest unit of life

3. All cells come from pre-existing cells

Magnification

How much larger the picture is than the real size of the specimen

HPFD/LPFD = LPM/HPM (how to find fov on high power)

formula you need to know

Scale = size of object in diagram/actual size of object (must be in the same units)

formula you need to know

Contrast

The difference in colour/light between the specimen and its background; it allows us to see depth.

Light Microscopes

Light passes through the specimen. It is cheaper compared to most microscops, small, and easy to use. Although, it has low magnitude, and it is hard to view live specimen.

Confocal Microscope

Uses lasers and computers to focus on a small area. It has highe resoultion, 3D imagery, and can view live specimen. Although, it is quite expensive, large and has low magnification.

Scanning Electron Microscope (SEM)

Beam of electrons is reflected off the specimen. It produces info about the surface of the specimen, can create 3D imagery, and has a high magnification (1 millionX). Although, its very large and expensive, does not view live specimen and is black and white.

Transmission Electron Microscope (TEM)

Beam of electrons is passes through the specimen. It produces info about the inner structure of the specimen, can create 3D imagery, and has a high magnification (1 millionX). Although, its very large and expensive, does not view live specimen and is black and white.