Glycolysis

Step 1

Glucose to glucose-6phosphate

Step 1 - enzyme

hexokinase

In which steps is ATP USED

1 + 3

Step 2

Glucose 6-phosphate to fructose 6-phosphate

Step 2 enzyme

phosphoglucose isomerase

Step 3

Fructose 6-phoshate to Fructose 1,6-biphosphate

Step 3 enzyme

phosphofructokinase

Step 4

Fructose 1,6-biphosphate splits to form glyceraldehyde 3-phosphate + dihydroxyacetone phosphate

Step 4 enzyme

aldolase

Step 5

Dihydroxyacetone phosphate rearranges to form glyceraldehyde 3-phosphate

Step 5 enzyme

triose phosphate isomerase

Step 6 (Energy pay off phase begins)

Glyceraldehyde 3-phosphate to 1,3-biphosphogylcerate

Step 6 enzyme

glyceraldehyde-3-phosphate dehydrogenase

At what step does 2 Pi + 2 NAD+ convert to 2 NADH and 2 H+

Step 6

Step 7

1,3-biphosphogylcerate to 3-phosphogylcerte

Step 7 enzyme

phosphoglycerate kinase

Step 8

3-phosphogylcerate to 2-phosphoglyerate

Step 8 enzyme

phosphoglycerate

At what step is H2O (2) produced

9

Step 9

2-phosphoglycerate to phosphoenolpyruvate

Step 9 enzyme

Enolase

Step 10

phosphoenolpyruvate to pyruvate (2)

Step 10 enzyme

pyruvate Kinase

At what step is ATP Produced

7 and 10

What steps in the reaction “capture energy”

6(NADH)+ 7(ATP) + 10 (ATP)

Which reactions are considered “substrate level phosphorylation”

7 + 10