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4 steps of the origins of life
formation of organic molecules (reducing atmosphere hypothesis, deep sea vent hypothesis, extraterrestrial hypothesis)
transformation of simple organic molecules into complex molecules (likely aided by mineral surfaces like clay that catalyze polymerization)
becoming cellular structures, separating internal from external (protobiont)
gaining chemical activity (RNA thought to be the first macromolecule in protobionts bc it can self-reproduce, catalyze, and store info
Miller-Urey experiment
Miller-Urey wanted to see if organic molecules could have formed in prebiotic conditions. They had O2, H2O, CH4, and NH3 in a thing that replicated prebiotic conditions and they waited to see if organic molecules would form in those conditions. They found that organic molecules were formed in those environments.
Fossil dating
technique used to determine specimen age by measuring percentage of radioisotope decay, igenous rocks
Evidence of evolution
homologies
selective breeding
fossils
vestigial structures
convergent evolution
what are homologs
features in different species that come from a shared common ancestor
paralogs vs orthologs
both homologs (genes come from common ancestor) but differ in how they evolved. Paralogs evolved via a gene duplication event and orthologs evolved through a speciation event
mechanisms of reproductive isolation
pre-zygotic - temporal isolation (awake at different times), geographic isolation (live in different places), behavioral isolation (having different sexual mating calls), gametic isolation (can have sex but cannot reproduce), mechanical isolation (physically cannot reproduce i.e. having diff genetalia)
post zygotic - hybrid insterility (can reproduce but the offspring is infertile) or hybrid inviability (zygote becomes inviable post fertilization)
speciation
evolutionary process in which populations evolve to become distinct species
bacterial vs archeal cell membranes
unbranched, ester bonded, D-glycerol, fatty acid chain = bacterial
branched, ether bonded, L-glycerol, isoprene chain = archeal
Horizontal gene transfer and mechanisms by which it occurs
Gram positive vs gram negative bacteria
have peptidoglycan layer with no envelope, purple in color (gram positive)
thinner peptidoglycan layer with outer envelope, Pink in color (gram negative)
Definition of protists
eukaryotes that don’t fit in animal plant or fungi kingdoms, can be uni or multicellular, like moist habitats, labelled by their ecological roles and motility
List Eukaryotic supergroups as covered in class
discoba
land plants and relatives
alveolata
stramenopila
rhizaria
ameobozoa
opsithokonta
Fungal phylogenetic tree
Plant phylogenetic tree
Alternation of generations (gametophyte and sporophyte stages and prominence in lifecycles)
Flower parts, perfect/imperfect flowers, incomplete/complete flowers
Monoecious vs dioecious plants
Angiosperm reproduction
Double fertilization
2 distinct fertilization events
Mycorrhizae and symbioses
Nitrogen fixing
Domains of life
importance of plant transport
for plant - growth, behavior, nutrition
for earth - climate, water cycle
difference between active and passive transport
passive doesnt use energy and active does
types of passive transport
passive diffusion - moves across membrane via concentration gradient
facilitated diffusion - protein in membrane moves it across membrane along gradient
aquaporins - facilitated diffusion of water
types of active transport
1 active transport - requires ATP, moves against gradient
2 active transport - ATP used to pump ions against gradient (H+ or NO3)
symporters - transports 2 substances in same direction across membrane
what type of transport do plants mainly use
active transport (bringing water and minerals up)
difference between hypotonic, hypertonic, and isotonic
hypotonic - lower solute outside the cell rather than inside
hypertonic - lower solute inside the cell rather than outside
isotonic - equal solute concentration inside and outside
how does a cell react in different solute concentrations
in hypotonic the cell becomes turgid (plasma membrane presses tightly against cell wall)
in hypertonic the cell becomes plasmolyzed (so much water loss that the membrane contorts away from wall)
in isotonic the cell becomes flaccid (the plasma membrane does not press tightly against cell wall)
water potential
tendency of water to move, moves from high to low potential
what are different adaptations to osmotic stress
osmotic adjustment - higher solute concentration of cytosol, lower water potential = water moves in
desiccation tolerance - sugars in cytosol bind to membrane = stabilization
halophytes - prevents water loss, increase inorganic salts in vacuoles, some excrete salt.
what is a symplast
continuum of cytosol linked by plasmodesmata
what is an apoplast
water-filled cell walls and intercellular spaces
what is symplastic
when molecules move to cytosol of an adjacent cell through plasmodesmata
what is apoplastic
when molecules move in water-filled cell walls and intercellular spaces
what is transmembrane
when molecules are exported out of 1 cell via membrane proteins and taken up by an adjacent cell
where does apoplastic stop
root endodermis
what is the casparian strip
creates a barrier to harmful chemicals so they don’t spread throughout the plant and kill the xylem
what is a process of long-distance transport and define it
bulk flow: mass movement of liquid by pressure, gravity, or both
xylem general information
has tracheary elements: tracheids & vessel elements
developement: 2nd wall deposited inside 1st wall, 2 wall is rich in lignin
lignin: for strength, durability, and water proofing
what are tracheids
long and narrow, usually slanted end walls, long tubes that fit together, end walls and pits are not lignified
what happens to tissues expand in the tracheids
the lignin starts spiraling
vessel elements
have pits inside walls, perforated end walls, lignified 2nd walls
what kind of plant are vessel elements located in
mainly angiosperms not gymnosperms
are vessel elements more efficient than tracheids and why
vessel elements because they have larger diameter which is better for bulk flow and the perforated end walls allows for faster flow
what is guttation and what causes it
when drops of liquid form outside plant due to root pressure
what is transpiration and when does it happen
the movement of water out the leaf via stomata, occurs when leaf is exposed to drier air
cohesion
water molecules sticking together, allows for continuous movement within the xylem
tension
when water evaporates surface tension increases in the intercellular spaces of cells
what are adaptations to water loss
waxy cuticles
stomatal opening and closing
leaf abscission - process of leaf shedding
how do organic molecules transport in phloem
sieve tube elements
companion cells
phloem parts
supporting fibers
parenchyma cells
sieve tube elements
companion cells
sieve tube elements
lost most cytoplasm and nucleus, arranged end to end
mature sieve tube elements
retains only peripheral cytoplasm, some ER, mitochondria, and plastids
sieve plates
perpherated end walls of mature sieve elements (perpherations are called sieve plate pores)
companion cells
support systems, provides mRNA and proteins to sieve tube elements
what is phloem loading
sugars move to companion cells or sieve tube elements via plasmodesmata
pressure flow hypothesis (phloem transport)
sugars load into phloem causes water to enter by bulk flow from xylem, water entry increases pressure causing sap to flow, accumulation of sugar reduces solute concentration resulting in bulk water flow into xylem and then water from xylem goes up via transpiration
behavior
response to stimulus (can be either external or internal)
examples of internal stimuli
plants produce chemical signals and hormones
examples of external stimuli
touch, wind, temp, gravity, soil, herbivores, symbionts, pathogens
trophism
growth in specific direction, dependent on stimulus (plant grows towards sun)
how does stimuli lead to behavioral activation
receptor molecules recieve signals and become activated, second messengers can be produced that transmit those signals to effector proteins, effector proteins affect gene expression or other cellular processes that can influence the plants behavior/development
Auxin function
establishes apical basil polarity, induces vascular tissue development, mediates phototropism, promotes formation of roots, inhibits leaf and fruit drops, stimulates fruit development
cytokinins function
promotes cell division, influences cell specialization and cell aging, activates secondary meristem developement, promotes root growth, promotes shoot development
gibberellins
stimulates cell division and cell elongation, stimulates stem elongation and flowering, promotes seed germination
abscisic acid
slows or stops metabolism during environmental stress, induces bud and seed dormancy, prevents seed germination in unfavorable conditions, promotes stomatal closing
how does auxin work in the cell
diffuses into cell as uncharged form or as an anion through auxin influx carrier, exits cell as an anion through auxin efflux carriers, locations of the auxin proteins determines the direction of the auxin flow
forms of auxin and what they are
IAAH: uncharged form
IAA-: charged form
AUX1: auxin influx carrier
PINS: auxin efflux carrier
hormones that deal with environmental stress
ABA, salicylic acid, systemic, jasmonic acid, NO
what is ABA
stops metabolism when conditions are poor, prevents seed germination and induces seed and bud dormancy
photoperiodism
ability to measure and respond to light amounts and day lengths
types of photoreceptors
blue light —> phototropins and cryptochromes
red light —> phytochromes
phytochromes
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how do plants protect from herbivores
when mouth secretions from herbivores and tissue damage induce production of toxin, toxin is released from plant as protection
sequence of steps of herbivore protection
herbivore damage : system in release : jasmonic acid release : goes up phloem : defensive chemical
what are the types of photoperiodism
long day - short nights, flowers in early spring
short day - long nights, flowers in late summer, fall or winter
day neutral - flower as long as minimum day length is met
types of plant insect interactions
co-evolution (flower and pollinator)
herbivores/plant chemicals
carnivorous plants
animal characteristics (some exceptions)
multicellular organisms, lack cell walls, heterotrophs (use other organisms for food), nervous tissue, movement, sexual reproduction, extracellular matrix (proteins bind animal cells together to give extra support and strength), characteristic cell junctions (specialized protein strucutres that connect adjacent cells, animals can have anchoring, tight, and gap junctions), special clusters of hox genes (function in patterning body axis), similar RNA
Choanoflaggellate ancestor
thought that most recent common ancestor was similar to choanoflagellates and similar to cells in sponges in the opisthokonta supergroup
animals are classified based on…
presence or absence of different tissue types, body symmetry, features of embryonic development
what are tissues
an association of many cells of the same type
metazoa
all animals
parazoa
sponges (and animals)
eumetazoa
true tissues
types of symmetry and define
radial - can make equal halves if cut in many different ways (jellyfish)
bilateral - can only make 2 equal halves if cut in one way (humans)
what is a diploblast and a triploblast
diploblast - 2 tissue layers when embryo
triploblast - 3 tissue layers when embryo
stages of embryonic development
cleavage leads to the formation of a hallow ball of cells called the blastula
gastrulation involves invagination of the blastula which creates the gastrula
in the gastrula layers of cells become the endoderm, the outside cells of the blastula form the ectoderm and a middle layer called the mesoderm is formed
protosomes
when the blastopore becomes a mouth
deuterostomes
when the blastopore becomes the anus
what are all the groups of animals
kingdom animalia, metazoa, parazoa, eumetazoa, radiata, bilateria, protosomia, deuterozoa, lophotrochozoa, ecdysozoa
what group does a protosome fall under
lophotrochozoa
what is a coelom
a body cavity lined on all sides by mesoderm derived tissue
segmentation
division of structures (worm body)
tissue types and where they come from
muscle tissue (muscle cell)
nervous tissue (neurons)
epithelial tissue (epithelial cell)
connective tissue (connective cell)
steps to make an organism
differentiated cell →tissue →organ →organ system →organism
types of muscular tissue
skeletal, smooth, cardiac
functions of the different muscular tissue
skeletal - attatched to bone in vertabrates and provide force needed for locomotion, under voluntary control, striated/striped
smooth - often surround hollow tubes, control tubes diameter, involuntary muscle contraction
cardiac - only found in heart where muscle cells are interconnected and give force for heartbeat, involuntary muscle contractions
main functions of epithelial tissue + 1 example
protection, secretion, absorption + urinary system or lungs