Microbiology Z125 Experiment 9

Vocabulary flashcards covering the enzymes, growth conditions, and media used in Experiment 9 of the Microbiology Z125 course.

Experiment 9 Incubation Parameters

The standard environment for growing cultures in this experiment is $37^{\circ}C$ for $18-24h$.

Lactase

An enzyme of interest in Part A (Carbohydrate digestion) used by E. coli to break down lactose.

Sucrase

An enzyme of interest in Part A (Carbohydrate digestion) used to break down sucrose into its products.

Amylase

The enzyme of interest in Part B responsible for starch digestion on a Starch Agar plate.

DNAase

The enzyme of interest in Part C that breaks down DNA, tested using a DNA plate with organisms such as PA, Ca, and Sm.

TSA Slant

Tryptic Soy Agar slant used in Part D to test for Catalase activity in organisms like E. coli and B.m.

SIM Tubes

Specialized medium used in Part E to test for Sulfur-indole-motility (SIM) using an inoculating needle.

Cysteine desulfurase

One of the enzymes of interest in Part E (SIM test) involved in sulfur reduction.

Tryptophanase

An enzyme of interest in Part E (SIM test) involved in indole production from the breakdown of tryptophan.

Urease

The enzyme of interest in Part F, tested by gliding an inoculating loop along the agar surface to observe breakdown products.

Starch Agar Plate

The specific medium used in Part B to detect starch digestion by amylase-producing organisms.

Glucase

As noted in Part A of the experiment, an enzyme of interest related to the breakdown products of glucose.