Amino Acids & Proteins

initially…. then….

initially negative then becomes positive

C—→ D ——>A—→ B

A. When the epitope (where antibody binds)of interest involves amino acids that are far apart in the primary structure

B. When the epitope of interest involves amino acids that are adjacent to each other in the primary structure

Answer: A

southwestern blot for protein (TF) to Dna inetarction

Answer: B

A. mutant containing only domain A binds the ligand with teh same Kd as wild type

B. mutant containly only B and C binds wiht a lower Kd than wild type

D. mutant with only B and C cannot bind the ligand

Answer: A

1 and 3 only

Answer B

Passage A

Passage A

Answer B

Passage A

Answer D

Passage A

A. sb facilitates ubiquitination of IRS4

B. sb induces IRS4 expression by entering the nucleus to bind DNA

C. sb domain prevents degradation of IRS4 by inhibiting proteases

D. sb domain becomes ubiquitinated, causing IRS4 to enter lysosome

answer A;

Met, Asn, Thr, Ser?

Answer: Met

Lys, Arg, Gln, Asp?

Gln