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Principle of Thermal Preservation
Regulated processes using heat that are performed commercially to control chemical, enzymatic and microbial reactions
3 Types of Thermal Preservation
Blanching
Pasteurization
Commerical Sterilization
Blanching
Exposing produce to boiling water/steam for a short period of time
To inactivate endogenous enzymes and drive off gases
Pasteurization
Boiling below boiling point of water (60-80°C)
low acid and acid foods
Pasteurizing Low Acid foods
Milk & Eggs
to destroy pathogenic (disease-causing) bacteria & viruses
to inactivate milk and enzymes
Pasteurizing Acid foods
Beer, wine, juice
To extend product-shelf life
Destroys spoilage-causing MOs & enzymes
Commercial Sterilization (CS)
Canning
Requires minimum of 131°C heat for 15 mins
destroys spoilage causing & pathogenic MOs
ensures Clostridium Botulinum are destroyed
Clostridium botulinum (C. botulinum)
Bacteria present in soil, water, air
Anaerobic (NO oxygen)
Thrives in low-acid foods
What can C. botulinum do?
produces a neurotoxin that affects the central nervous system
results in vertigo, blurred vision, difficulty breathing & swallowing, slurred speech
worst cases: death from respiratory collapse or cardiac arrest
Characteristics of CS containers
CS conducted after packaging
must withstand high temp and pressure
hermetically sealed / airtight
impermeable by gases, liquids, and microorganisms
shelf life of 2+ years
UHT processing and Aseptic Packaging = Commercial Sterilization (what are they)
UHT: injection of hot steam under pressure (140 - 150°C) for a short period of time (4-6 secs); followed by immediate cooling
Aseptic packaging: commercially sterilized product is placed into pre-sterilized container and sealed in an aseptic environment
ex. shelf-stable milk, juice
Why select CS?
longer storage time at room temp
Goal of Selecting Heat Treatments (HT)
To minimize nutrient loss and ensure appropriate storage
Sufficient & Mild Treatments
Why select Sufficient Heat for treatment?
To destroy microorganisms & food enzymes
Mildest HT
Guarantees freedom from pathogens and toxins while producing desired storage life
Milk Processing Process (3)
Clarification
Homogenization
Pasteurization
Clarification
Raw milk is passed through an industrial centrifugal clarifier
Purpose:
To remove impurities that may have accidentally entered the milk at farm level, ensuring it is physically clean before chemical processing
Homogenization
The milk is pumped under extreme physical pressure through a microscopic valve
Purpose:
To break down the large fat (cream) into smaller droplets
Shrinking the size of the droplets prevents the cream from spreading and rising to the top of the container during storage, providing a smooth, consistent mouthfeel
Pasteurization
A moderate-intensity thermal process that heats fluid milk to a specific temperature below boiling point
Purpose:
The primary safety hurdle to completely destroy pathogenic bacteria and viruses
Inactivates naturally occurring enzymes to prevent spoilage
Pasteurization of dairy products other than milk- the effect of other ingredients (e.g. in egg nog)
Other ingredients in pasteurization of dairy products other than milk affect require a higher temperature and holding time
Sugars, starches and fats protect bacteria from heat
Tetra Pak
Container with a Hermetic seal and a 7 layers
Thermal Death Curves (TDC)
graphs used by the food industry to determine how much treatment a food needs to be safe and high quality
Microorganisms are not killed instantly when exposed to heat, but instead die in a predictable logarithmic order of death
Under constant thermal conditions, the same % of microbial population will be destroyed in a given time interval, regardless of size
2 Types of Thermal Death Curves
Thermal Death Rate Curves (TDRC)
Thermal Death Time Curves (TDTC)
Thermal Death Rate Curves (TDRC)
graph that looks at a microbial population heated at one specific, constant temperature (ex. 121°C)
one specific temperature that allows you to calculate the D-value by measuring how much time it takes for the population to drop
D-Value (decimal reduction time)
time (mins) at a particular temp required to kill 90% of a microbial population
If u increase the temp, the time required to kill the pathogen is decreased
Thermal Death Time Curves (TDTC)
time required for destruction of a microbe under specific conditions at different temperatures
z-value (°C) and F-value (min)
Z-Value
Allows a comparison of heating processes at different temperatures because in the real world, we don’t have instant heat at 121ºC
Resistance of MO to temperature Variation
F-Value
Time (min) required ot kill MOs at 121ºC; lethality of heat treatment
Margin of Safety (MS)
guarantees that even if a food is heavily contaminated with highly dangerous microbial spores, the heat treatment will thoroughly destroy them
probability of surviving C. botulinum spores
intention is to have a wide margin of safety to minimize the probability of survivors
MS in Low Acid foods
12D thermal process
MS in Acid Foods
5D thermal process
How can we determine if C. botulinum spores have been destroyed?
Inoculated pack studies
Clostridium sporogenes PA3679
Inoculated pack studies
Experiment to check if a canning process (time & temp) works
scientists contaminate (inoculate) a test batch with harmless bacterial spores
run these test cans through industrial cooker, incubate, and see if any spores survive
if spores are 100% dead, heat treatment is setup and safe
Clostridium sporogenes PA3679
Harmless strain of bacterium that is a putrefactive anaerobe
more heat resistant than C. botulinum, so they require a higher temp an time
The “Cold Point”
When a sealed can is cooked, the heat travels from the outside walls inward
The cold point is the absolute last part of the container to reach the required sterilization temperature
Temperature sensors must be placed directly at the cold point because if this spot reaches the safety target, everything else in the can is fine
location determined by the mechanism of heat transfer
Mechanism of heat transfer is determined by (2)
Consistency of Food
Chemical Composition
Consistency of Food