Histotechnique Basics

Flashcards covering the fundamental requirements, materials, and stages of tissue processing in histotechnique.

Histotechnique

A process that necessitates the cutting of thin sections of fixed tissues.

Required Tissue State

Fixed tissues must be of suitable hardness and Consistency when presented to the knife edge to allow thin sections to be cut.

Infiltrating and Surrounding (Embedding)

Methods used to impart desired haukss and Consistency to tissue using materials like paraffin wax, celloiding, LVM, or resins.

LVM

how viscocity nitrocellulose used as an embedding medium to provide hardness to fixed tissues.

Paraffin wax

Material used for embedding practically all routine chegnostic block.

Processing

The term used to describe the various stages between fixation and cutting of Sections.

Prerequisites for Infiltration

Processing is only possible after the Fixed tissues has been dehydrated and cleared.

Stage 1 of Processing

Dehydration.

Stage 2 of Processing

Clearing.

Stage 3 of Processing

Infiltration and Embedding.