capillary electrophoresis

electropherogram

In CE, chromatogram is…

applied potential

In CE, flow rate is…

carrier electrolyte (buffer)

In CE, mobile phase is…

injection mode

In CE, injector is…

migration time

In CE, retention time is…

electrophoretic mobility

In CE, column capacity factor is…

high voltage power supply

In CE, pump is…

capillary

In CE, column is…

1. minimal sample consumption and waste production

2. high separation efficiency

3. rapid analysis and reduced run times

4. excellent for charged molecules and fast separations

4 advantages of CE

due to microscale dimensions and uniform flow front

Why does CE have a high separation efficiency?

1. limited capacity for separating large molecules

2. may require specialized equipment for some applications

3. sensitive to changes in buffer composition

3 disadvantages of CE

1. charged or ionic analytes

2. speed is crucial

3. high separation efficiency of small molecules is required

4. minimal sample consumption is required

4 times to choose CE over LC

1. analytes vary in size, charge, and hydrophobicity

2. superior separation control and flexibility are needed

3. analyzing complex sample with diverse range of compounds

4. longer separation times are acceptable

4 times to choose LC over CE

electrophoretic mobility and electroosmotic flow

2 factors that cause mobility of solutes in CE

electrophoretic mobility

movement of a charge species under the influence of an applied electric field

cathode; anode

in electrophoretic mobility, cations migrate towards the ___ and anions towards the ___

electroosmotic flow

migration of solutes in response to the buffer solution’s movement in response to an electric field

buffer; cathode

in electroosmotic flow, the ___ moves towards the ___ and sweeps all species in that direction including anions and neutrals

1. charge density of solute

2. dielectric constant and viscosity of electrolyte

3. temperature

3 things electrophoretic mobility depends on