LAB

What process was used to collect cheek cells for isolating the TAS2R38 gene?

Cheek cells were collected by rinsing with saline and centrifuging the solution to form a cell pellet.

What method was used to release the DNA from the cheek cells?

Heat and Chelex were used to lyse the cells, rupturing the cell and nuclear membranes to release DNA.

What is the purpose of PCR in the isolation of the TAS2R38 gene?

PCR is used to amplify the target TAS2R38 gene, exponentially increasing the quantity of a specific DNA region.

How do restriction enzymes function in relation to the TAS2R38 gene?

Restriction enzymes cut the amplified TAS2R38 DNA segments into pieces based on specific SNPs present in the individual's nucleotide composition.

What visual method is employed to analyze the DNA segments post-restriction enzyme treatment?

Gel Electrophoresis is used to visualize the resulting TAS2R38 segments.

What DNA pattern results from a homozygous non-taster regarding TAS2R38?

A homozygous non-taster will result in one large DNA cluster.

What DNA pattern is observed for a homozygous taster of the TAS2R38 gene?

A homozygous taster will result in two distinct DNA clusters.

How many separate DNA clusters are observed in a heterozygous taster of TAS2R38?

A heterozygous taster will result in three separate clusters of DNAs.